Overview

  • Product nameAnti-Talin 1 and 2 antibody [8D4]
    See all Talin 1 and 2 primary antibodies
  • Description
    Mouse monoclonal [8D4] to Talin 1 and 2
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, ICC, IPmore details
  • Species reactivity
    Reacts with: Rat, Human, Xenopus laevis
    Does not react with: Fish
  • Immunogen

    Cytoskeletal protein talin, purified from chicken gizzard.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR228677. Lot numbers higher than GR228677 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.097% Sodium azide
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone number8D4
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100. See Abreview.
Flow Cyt 1/250. (see Abreview).



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 225 kDa.

Use at an assay dependent concentration.

ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • RelevanceTalin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations. Talin 2 is related to talin 1. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton.
  • Cellular localizationCell projection, ruffle membrane (talin 1), Focal adhesion plaques and synapses (talin 2)
  • Database links
  • Alternative names
    • talin 1 antibody
    • talin 2 antibody
    • talin antibody
    • talin1 antibody
    • talin2 antibody
    • TLN antibody
    • TLN1 antibody
    • TLN2 antibody
    see all

Anti-Talin 1 and 2 antibody [8D4] images

  • Central Panel: ab11188 staining Talin 1 and 2 in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.
    Left-hand panel: Nuclei counterstained with DAPI (blue).
    Right-hand panel: Overlay

    See Abreview

  • ab11188 staining Talin 1 and 2 in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated goat anti-mouse IgG (H+L) at a 1/500 dilution.

    P : Permeabilized;
    US : Unstained, Red Peak;
    IGG MS : IgG Mouse (Isotype Control), Blue Peak;
    Talin, Green peak.

    See Abreview

References for Anti-Talin 1 and 2 antibody [8D4] (ab11188)

This product has been referenced in:
  • Case LB  et al. Molecular mechanism of vinculin activation and nanoscale spatial organization in focal adhesions. Nat Cell Biol 17:880-92 (2015). WB ; Human . Read more (PubMed: 26053221) »
  • Granato DC  et al. Integrated proteomics identified up-regulated focal adhesion-mediated proteins in human squamous cell carcinoma in an orthotopic murine model. PLoS One 9:e98208 (2014). WB, IHC-P ; Human . Read more (PubMed: 24858105) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (rat benign mammary cell line)
Loading amount 20 µg
Specification rat benign mammary cell line
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment ADP for 30 min
Gel Running Conditions Reduced Denaturing (8-16% Biorad Criterion Gel with 18 wells)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy Platelets
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (PMN)
Specification PMN
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX-100 + 2% BSA in PBS for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"