Overview

  • Product nameAnti-Talin 1 antibody [1A11]
    See all Talin 1 primary antibodies
  • Description
    Mouse monoclonal [1A11] to Talin 1
  • Tested applicationsSuitable for: Sandwich ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1052-1150 of Human Talin 1 with proprietary tag (NP_006280).

  • Positive control
    • Human Spleen and Testis tissue; PC-12 and Raw 264.7 cell lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number1A11
  • IsotypeIgG3
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged TLN1 is approximately 0.3 ng/ml as a capture antibody.
WB Use at an assay dependent dilution. Predicted molecular weight: 269 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
  • Sequence similaritiesContains 1 FERM domain.
    Contains 1 I/LWEQ domain.
  • Cellular localizationCell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • ILWEQ antibody
    • Talin 1 antibody
    • Talin antibody
    • Talin-1 antibody
    • TLN 1 antibody
    • TLN antibody
    • Tln1 antibody
    • TLN1_HUMAN antibody
    see all

Anti-Talin 1 antibody [1A11] images

  • ab110080, at 5µg/ml, staining Talin 1 in formalin-fixed, paraffin-embedded Human Spleen tissue by Immunohistochemistry.
  • ab110080, at 5µg/ml, staining Talin 1 in formalin-fixed, paraffin-embedded Human Testis tissue by Immunohistochemistry.
  • Anti-Talin 1 antibody [1A11] (ab110080) + PC-12 cell lysate

    Predicted band size : 269 kDa
  • Anti-Talin 1 antibody [1A11] (ab110080) + Raw 264.7 cell lysate

    Predicted band size : 269 kDa
  • Detection limit for recombinant tagged Talin 1 is approximately 0.3 ng/ml as a capture antibody.

References for Anti-Talin 1 antibody [1A11] (ab110080)

ab110080 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"