Overview

  • Product name
    Anti-Talin 1 antibody [1A11]
    See all Talin 1 primary antibodies
  • Description
    Mouse monoclonal [1A11] to Talin 1
  • Tested applications
    Suitable for: Sandwich ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1052-1150 of Human Talin 1 with proprietary tag (NP_006280).

  • Positive control
    • Human Spleen and Testis tissue; PC-12 and Raw 264.7 cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    1A11
  • Isotype
    IgG3
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged TLN1 is approximately 0.3 ng/ml as a capture antibody.
WB Use at an assay dependent dilution. Predicted molecular weight: 269 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
  • Sequence similarities
    Contains 1 FERM domain.
    Contains 1 I/LWEQ domain.
  • Cellular localization
    Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • ILWEQ antibody
    • Talin 1 antibody
    • Talin antibody
    • Talin-1 antibody
    • TLN 1 antibody
    • TLN antibody
    • Tln1 antibody
    • TLN1_HUMAN antibody
    see all

Anti-Talin 1 antibody [1A11] images

  • ab110080, at 5µg/ml, staining Talin 1 in formalin-fixed, paraffin-embedded Human Spleen tissue by Immunohistochemistry.
  • ab110080, at 5µg/ml, staining Talin 1 in formalin-fixed, paraffin-embedded Human Testis tissue by Immunohistochemistry.
  • Anti-Talin 1 antibody [1A11] (ab110080) + PC-12 cell lysate

    Predicted band size : 269 kDa
  • Anti-Talin 1 antibody [1A11] (ab110080) + Raw 264.7 cell lysate

    Predicted band size : 269 kDa
  • Detection limit for recombinant tagged Talin 1 is approximately 0.3 ng/ml as a capture antibody.

References for Anti-Talin 1 antibody [1A11] (ab110080)

ab110080 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab110080.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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