The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detection limit for recombinant tagged TLN1 is approximately 0.3 ng/ml as a capture antibody.
Use at an assay dependent dilution. Predicted molecular weight: 269 kDa.
Use a concentration of 5 µg/ml.
FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
Sequence similaritiesContains 1 FERM domain. Contains 1 I/LWEQ domain.
Cellular localizationCell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity).