Overview

  • Product name
    Anti-Talin 1 antibody [93E12]
    See all Talin 1 primary antibodies
  • Description
    Mouse monoclonal [93E12] to Talin 1
  • Specificity
    ab104913 can be used for specific detection of the focal adhesion protein Talin 1 and does not cross react with Talin 2.
  • Tested applications
    Suitable for: ELISA, ICC/IF, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 780-850 of Talin 1.

  • Positive control
    • Fibroblasts
  • General notes


    Growth Conditions: RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37C + 5%CO2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    Growth Conditions: RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37C + 5%CO2.
  • Clonality
    Monoclonal
  • Clone number
    93E12
  • Myeloma
    NS0/1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/1000.
WB 1/100 - 1/1000. Predicted molecular weight: 270 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
  • Sequence similarities
    Contains 1 FERM domain.
    Contains 1 I/LWEQ domain.
  • Cellular localization
    Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • ILWEQ antibody
    • Talin 1 antibody
    • Talin antibody
    • Talin-1 antibody
    • TLN 1 antibody
    • TLN antibody
    • Tln1 antibody
    • TLN1_HUMAN antibody
    see all

References for Anti-Talin 1 antibody [93E12] (ab104913)

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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