Overview

  • Product nameAnti-Talin 1 antibody [93E12]
    See all Talin 1 primary antibodies
  • Description
    Mouse monoclonal [93E12] to Talin 1
  • Specificityab104913 can be used for specific detection of the focal adhesion protein Talin 1 and does not cross react with Talin 2.
  • Tested applicationsSuitable for: ELISA, ICC/IF, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 780-850 of Talin 1.

  • Positive control
    • Fibroblasts
  • General notes


    Growth Conditions: RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37C + 5%CO2.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesGrowth Conditions: RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37C + 5%CO2.
  • ClonalityMonoclonal
  • Clone number93E12
  • MyelomaNS0/1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/1000.
WB 1/100 - 1/1000. Predicted molecular weight: 270 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
  • Sequence similaritiesContains 1 FERM domain.
    Contains 1 I/LWEQ domain.
  • Cellular localizationCell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • ILWEQ antibody
    • Talin 1 antibody
    • Talin antibody
    • Talin-1 antibody
    • TLN 1 antibody
    • TLN antibody
    • Tln1 antibody
    • TLN1_HUMAN antibody
    see all

References for Anti-Talin 1 antibody [93E12] (ab104913)

ab104913 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"