Synthetic peptide conjugated to KLH derived from within residues 1650 - 1750 of Human Talin 1.
(Peptide available as ab71332.)
Our Abpromise guarantee covers the use of ab71333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 270 kDa (predicted molecular weight: 270 kDa).Can be blocked with Human Talin 1 peptide (ab71332).|
|Flow Cyt||Use a concentration of 3 - 4 µg/ml. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Sample: mouse endoderm cell
Fix in 3% PFA in PBS for 30 min at RT
Primary antibody: Rabbit anti-talin (ab71333), 1:100
Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3® 488) preadsorbed (ab97075), 1:100
Nuclear counterstain: DAPI
Nuclei were counterstained with DAPI
This image is courtesy of an anonymous abreview.Detection method: Licor System