Our Abpromise guarantee covers the use of ab171120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 10 µg/mg of lysate.|
Immunoprecipitation was performed on purified TAMRA-BSA. The antigen:antibody complex was formed by binding 5 µg protein with 10 µg of ab171120 or mouse IgG, whole molecule overnight at 4°C. The immune-complex was then captured on 50 µl Protein A/G Plus Agarose. Captured immune-complexes were then washes extensively and proteins eluted with sample loading dye. Samples were resolved on a SDS PAGE gel.
Lane 1: ab171120
Lane 2: BSA
Lane 3: TAMRA-BSA
Lane 4: BSA-ab171120
Lane 5: TAMRA-BSA + mouse IgG
Lane 6: TAMRA-BSA + ab171120
ab171120 has not yet been referenced specifically in any publications.