Overview

  • Product name
    Anti-TAMRA antibody [5G5]
    See all TAMRA primary antibodies
  • Description
    Mouse monoclonal [5G5] to TAMRA
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IPmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to TAMRA.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    5G5
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IP Use at 10 µg/mg of lysate.

Target

  • Alternative names
    • 2-[3,6-bis(dimethylamino)xanthenium-9-yl]benzoate antibody
    • 2-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate antibody
    • CHEBI:52282 antibody
    • CHEMBL2074882 antibody
    • Tetramethylrhodamine antibody
    • ZINC05140632 antibody
    see all

Images

  • Immunoprecipitation was performed on purified TAMRA-BSA. The antigen:antibody complex was formed by binding 5 µg protein with 10 µg of ab171120 or mouse IgG, whole molecule overnight at 4°C. The immune-complex was then captured on 50 µl Protein A/G Plus Agarose. Captured immune-complexes were then washes extensively and proteins eluted with sample loading dye. Samples were resolved on a SDS PAGE gel.

    Lane 1: ab171120

    Lane 2: BSA

    Lane 3: TAMRA-BSA

    Lane 4: BSA-ab171120

    Lane 5: TAMRA-BSA + mouse IgG

    Lane 6: TAMRA-BSA + ab171120

     

     

  • All lanes : Anti-TAMRA antibody [5G5] (ab171120) at 1/1000 dilution

    Lane 1 : TAMRA-BSA at 10 µg
    Lane 2 : TAMRA-BSA at 5 µg
    Lane 3 : TAMRA-BSA at 2 µg
    Lane 4 : TAMRA-BSA at 1 µg
    Lane 5 : TAMRA-BSA at 0.5 µg
    Lane 6 : TAMRA-BSA at 0 µg

    Secondary
    All lanes : Goat anti-mouse-HRP

    Developed using the ECL technique.

References

ab171120 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171120.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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