Overview

  • Product name
  • Description
    Rabbit polyclonal to TANK
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab18819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 47 kDa.Can be blocked with Human TANK peptide (ab92908).
IP Use a concentration of 10 - 20 µg/ml.
IHC-Fr Use a concentration of 10 - 20 µg/ml.

Target

  • Function
    Acts as a regulator of TRAF function by maintaining them in a latent state. Overexpression inhibits TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. May be involved in I-kappa-B-kinase (IKK) regulation; may function as an adapter for kinases such as TBK1 or IKBKE that can modulate IKK activity.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated by IKBKE.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • I TRAF antibody
    • I-TRAF antibody
    • ITRAF antibody
    • Tank antibody
    • TANK_HUMAN antibody
    • TRAF family member associated NF KAPPA B activator antibody
    • TRAF family member associated NFKB activator antibody
    • TRAF family member-associated NF-kappa-B activator antibody
    • TRAF interacting protein antibody
    • TRAF interacting protein TANK isoform a antibody
    • TRAF interacting protein TANK isoform b antibody
    • TRAF-interacting protein antibody
    • TRAF2 antibody
    see all

Images

  • ICC/IF image of ab18819 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18819, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Unterholzner L  et al. Vaccinia virus protein C6 is a virulence factor that binds TBK-1 adaptor proteins and inhibits activation of IRF3 and IRF7. PLoS Pathog 7:e1002247 (2011). IP ; Human . Read more (PubMed: 21931555) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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