Overview

  • Product name
  • Description
    Rabbit polyclonal to TANK
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 14 amino acid peptide from near the carboxy terminus of human TANK.

  • Positive control
    • Daudi Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab47632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function
    Acts as a regulator of TRAF function by maintaining them in a latent state. Overexpression inhibits TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. May be involved in I-kappa-B-kinase (IKK) regulation; may function as an adapter for kinases such as TBK1 or IKBKE that can modulate IKK activity.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated by IKBKE.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • I TRAF antibody
    • I-TRAF antibody
    • ITRAF antibody
    • Tank antibody
    • TANK_HUMAN antibody
    • TRAF family member associated NF KAPPA B activator antibody
    • TRAF family member associated NFKB activator antibody
    • TRAF family member-associated NF-kappa-B activator antibody
    • TRAF interacting protein antibody
    • TRAF interacting protein TANK isoform a antibody
    • TRAF interacting protein TANK isoform b antibody
    • TRAF-interacting protein antibody
    • TRAF2 antibody
    see all

Images



  • Predicted band size : 47 kDa
    Western Blot of human kidney lysate labeling TANK with Anti-TANK antibody (ab47632) at (1) 1 and (2) 2µg/ml.
  • Lane 1 : Anti-TANK antibody (ab47632) at 0.5 µg/ml
    Lane 2 : Anti-TANK antibody (ab47632) at 1 µg/ml
    Lane 3 : Anti-TANK antibody (ab47632) at 2 µg/ml

    Lane 1 : Daudi Cell Lysate
    Lane 2 : Daudi Cell Lysate
    Lane 3 : Daudi Cell Lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of TANK in Rat Spleen cells using ab47632 at 20 ug/ml.

References

ab47632 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab47632.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up