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Rabbit polyclonal to TAOK3
Synthetic peptide corresponding to Human TAOK3 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q9H2K8
293 cell lysates transiently transfected with the JIK gene (WB)
Human hepatocarcinoma tissue (IHC)
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein A purified
ab62915 was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).
1/10 - 1/50.
Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK.
Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm. Cell membrane. Also localized to the peripheral cell membrane.
Information by UniProt
CTCL tumor antigen HD CL 09 antibody
CTCL-associated antigen HD-CL-09 antibody
Cutaneous T cell lymphoma tumor antigen HD CL 09 antibody
Western blot - Anti-TAOK3 antibody (ab62915)
All lanes : Anti-TAOK3 antibody (ab62915) at 1/50 dilution Lane 1 : 293 cell lysates nontransfected Lane 2 : 293 cell lysates transiently transfected with the JIK gene Lysates/proteins at 2 µg per lane. Predicted band size : 105 kDa Observed band size : 105 kDa Additional bands at : 60 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAOK3 antibody (ab62915)
ab62915, at 1/10 dilution, staining TAOK3 in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue by immunohistochemistry. ab62915 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
has not yet been referenced specifically in any publications.
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