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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Protein A purified
Purification notes ab62915 was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).
1/10 - 1/50.
Function Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK.
Tissue specificity Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.
Sequence similarities Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain.
Post-translational modifications Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization Cytoplasm. Cell membrane. Also localized to the peripheral cell membrane.
Information by UniProt
CTCL tumor antigen HD CL 09 antibody
CTCL-associated antigen HD-CL-09 antibody
Cutaneous T cell lymphoma tumor antigen HD CL 09 antibody
Anti-TAOK3 antibody images
Western blot - Anti-TAOK3 antibody (ab62915)
All lanes : Anti-TAOK3 antibody (ab62915) at 1/50 dilution Lane 1 : 293 cell lysates nontransfected Lane 2 : 293 cell lysates transiently transfected with the JIK gene Lysates/proteins at 2 µg per lane. Predicted band size : 105 kDa Observed band size : 105 kDa Additional bands at : 60 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAOK3 antibody (ab62915)
ab62915, at 1/10 dilution, staining TAOK3 in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue by immunohistochemistry. ab62915 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
References for Anti-TAOK3 antibody (ab62915)
has not yet been referenced specifically in any publications.
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