Anti-TAOK3 antibody (ab70297)


  • Product nameAnti-TAOK3 antibody
    See all TAOK3 primary antibodies
  • Description
    Rabbit polyclonal to TAOK3
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human TAOK3 (C terminal).
    Database link: Q9H2K8

  • Positive control
    • HeLa whole cell lysates.



Our Abpromise guarantee covers the use of ab70297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: Use at 1-5µg/mg of lysate.
    WB: 1/5000 - 1/25000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK.
    • Tissue specificityUbiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm. Cell membrane. Also localized to the peripheral cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CTCL tumor antigen HD CL 09 antibody
      • CTCL-associated antigen HD-CL-09 antibody
      • Cutaneous T cell lymphoma tumor antigen HD CL 09 antibody
      • Cutaneous T-cell lymphoma-associated antigen HD-CL-09 antibody
      • Dendritic cell derived protein kinase antibody
      • Dendritic cell-derived protein kinase antibody
      • DKFZp666H245 antibody
      • DPK antibody
      • FLJ31808 antibody
      • hKFC A antibody
      • hKFC-A antibody
      • JIK antibody
      • JNK/SAPK inhibitory kinase antibody
      • JNK/SAPK-inhibitory kinase antibody
      • Jun kinase inhibitory kinase antibody
      • Jun kinase-inhibitory kinase antibody
      • KDS antibody
      • Kinase from chicken homolog A antibody
      • MAP3K18 antibody
      • Serine kinase antibody
      • Serine/threonine-protein kinase TAO3 antibody
      • STE20 like kinase antibody
      • TAO kinase 3 antibody
      • TAOK3 antibody
      • TAOK3_HUMAN antibody
      • Thousand and one amino acid protein 3 antibody
      see all

    Anti-TAOK3 antibody images

    • All lanes : Anti-TAOK3 antibody (ab70297) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg

      Developed using the ECL technique

      Predicted band size : 105 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Western Blot showing Lane 2: ab70297 staining JIK in HeLa whole cell lysates immunoprecipitated using ab70297 at 3µg/mg lysate (1mg/lane). Lane 1: control IgG used for immunoprecipitation.Detection: Chemiluminescence with an exposure time of 3 minutes

    References for Anti-TAOK3 antibody (ab70297)

    ab70297 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Murine embryonic fibroblast)
    Gel Running Conditions Non-reduced Denaturing (gel 10%)
    Loading amount 20 µg
    Treatment siRNA trasnfection directed against TAOK3
    Specification Murine embryonic fibroblast
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Bastiaan Maes

    Verified customer

    Submitted May 02 2016

    Thank you for taking the time to complete our questionnaire as requested.

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    Application Western blot
    Sample Mouse Tissue lysate - whole (Spleen)
    Loading amount 100 µg
    Specification Spleen
    Gel Running Conditions Reduced Denaturing (6% running section)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted May 18 2009