KDS overexpression 293T lysate (whole cell) (ab94147)
Overview
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Product name
KDS overexpression 293T lysate (whole cell)
See all KDS lysates -
General notes
ab94147 is a 293T cell transfected lysate in which Human JIK has been transiently over-expressed using a pCMV-JIK. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Research areas
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Background
Function: Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK. Tissue specificity: Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain. PTM: Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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Notes |
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WB
Use at an assay dependent dilution. |
Images
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ab94147 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-KDS antibody (ab57359) at 1/500 dilution
Lane 1 :KDS overexpression 293T lysate (whole cell) (ab94147)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab94147 has not yet been referenced specifically in any publications.