Recombinant Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4883] to TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883]
See all TAOK2 + TAOK1 + TAOK3 primary antibodies -
Description
Rabbit monoclonal [EPR4883] to TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) -
Host species
Rabbit -
Specificity
ab124841 recognizes Human TAOK1 when phosphorylated at Serine 181, TAOK2 when phosphorylated at Serine 181 and TAOK3 when phosphorylated at Serine 177.
ab124841 cross reactivates with MEK1 due to high immunogen homology.
The antibody is detecting TAOK2 + TAOK1 + TAOK3 at around 138 kDa and MAP2K1 at around 44kDa.
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Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4883 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 138 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 138 kDa. |
Images
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All lanes : Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841) at 1/10000 dilution (Purified)
Lane 1 : Rat brain lysate
Lane 2 : Rat brain lysate, then the membrane treated with Lambda Phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 138 kDa
Observed band size: 110-140 kDa why is the actual band size different from the predicted?We are unsure about the nature of the extra bands. Bands at 110 to 140.
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All lanes : Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841) at 1/10000 dilution (Purified)
Lane 1 : Mouse brain lysate
Lane 2 : Mouse brain lysate, then the membrane treated with Lambda Phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 138 kDa
Observed band size: 110-140 kDa why is the actual band size different from the predicted?We are unsure about the nature of the extra bands. Bands at 110 to 140.
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All lanes : Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841) at 1/10000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate, then the membrane treated with Lambda Phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 138 kDa
Observed band size: 110-140 kDa why is the actual band size different from the predicted?We are unsure about the nature of other bands. Bands at 110 to 140.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab124841 has been referenced in 2 publications.
- Yesilkanal AE et al. Limited inhibition of multiple nodes in a driver network blocks metastasis. Elife 10:N/A (2021). PubMed: 33973518
- Hwang IY et al. Gai Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression. Front Immunol 9:687 (2018). PubMed: 29696016