• Product nameAnti-TAP1 antibody [53H8]
    See all TAP1 primary antibodies
  • Description
    Mouse monoclonal [53H8] to TAP1
  • Tested applicationsSuitable for: ELISA, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesProtein A purified from tissue culture supernatant.
  • ClonalityMonoclonal
  • Clone number53H8
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab10356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 81 kDa (predicted molecular weight: 87 kDa).
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.


  • FunctionInvolved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
  • Involvement in diseaseDefects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1) [MIM:604571]; also called HLA class I deficiency. BLS1 is a class I antigen deficiency that is not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.
  • DomainThe peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
  • Cellular localizationEndoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 17 antibody
    • ABC transporter MHC 1 antibody
    • ABC17 antibody
    • ABCB 2 antibody
    • ABCB2 antibody
    • Antigen peptide transporter 1 antibody
    • APT 1 antibody
    • APT1 antibody
    • ATP binding cassette sub family B (MDR/TAP) member 2 antibody
    • ATP binding cassette sub family B member 2 antibody
    • ATP binding cassette transporter antibody
    • ATP-binding cassette sub-family B member 2 antibody
    • D6S114E antibody
    • FLJ26666 antibody
    • FLJ41500 antibody
    • Peptide supply factor 1 antibody
    • Peptide transporter involved in antigen processing 1 antibody
    • Peptide transporter PSF 1 antibody
    • Peptide transporter PSF1 antibody
    • Peptide transporter TAP 1 antibody
    • Peptide transporter TAP1 antibody
    • PSF 1 antibody
    • PSF-1 antibody
    • PSF1 antibody
    • Really interesting new gene 4 protein antibody
    • RING 4 antibody
    • RING4 antibody
    • TAP 1 antibody
    • TAP1 antibody
    • TAP1*0102N antibody
    • TAP1_HUMAN antibody
    • TAP1N antibody
    • Transporter 1 ATP binding cassette sub family B (MDR/TAP) antibody
    • Transporter 1 ATP binding cassette sub family B antibody
    • Transporter associated with antigen processing antibody
    • Transporter ATP binding cassette major histocompatibility complex 1 antibody
    • Y3 antibody
    see all

Anti-TAP1 antibody [53H8] images

  • All lanes : Anti-TAP1 antibody [53H8] (ab10356) at 5 µg/ml

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 87 kDa
    Observed band size : 81 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa (possible non-specific binding).

References for Anti-TAP1 antibody [53H8] (ab10356)

ab10356 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Cervix)
Loading amount 20 µg
Specification Cervix
Gel Running Conditions Reduced Denaturing (12%)
Blocking step AECL (3% w/v) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C

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Submitted Nov 25 2008