Overview

  • Product name
  • Description
    Rabbit polyclonal to TAP1
  • Specificity
    This antibody detects an ~70 kDa protein, corresponding to the apparent molecular mass of Transporter Associated with Antigen Processing 1 (TAP1) on SDS-PAGE immunoblots. The immunogen sequence used to generate this antibody is based on the sequence of ATP-binding cassette sub-family B member 2.
  • Tested applications
    Suitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide:

    CYWAMVQAPADAPE

    , corresponding to amino acids 735-748 of Human TAP1 (member 2).

  • Positive control
    • HeLa cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: Whole Serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/4000. Detects a band of approximately 70 kDa.
IP 1/100.
ICC Use at an assay dependent dilution.

Target

  • Function
    Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
  • Involvement in disease
    Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1) [MIM:604571]; also called HLA class I deficiency. BLS1 is a class I antigen deficiency that is not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.
  • Domain
    The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
  • Cellular localization
    Endoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 17 antibody
    • ABC transporter MHC 1 antibody
    • ABC17 antibody
    • ABCB 2 antibody
    • ABCB2 antibody
    • Antigen peptide transporter 1 antibody
    • APT 1 antibody
    • APT1 antibody
    • ATP binding cassette sub family B (MDR/TAP) member 2 antibody
    • ATP binding cassette sub family B member 2 antibody
    • ATP binding cassette transporter antibody
    • ATP-binding cassette sub-family B member 2 antibody
    • D6S114E antibody
    • FLJ26666 antibody
    • FLJ41500 antibody
    • Peptide supply factor 1 antibody
    • Peptide transporter involved in antigen processing 1 antibody
    • Peptide transporter PSF 1 antibody
    • Peptide transporter PSF1 antibody
    • Peptide transporter TAP 1 antibody
    • Peptide transporter TAP1 antibody
    • PSF 1 antibody
    • PSF-1 antibody
    • PSF1 antibody
    • Really interesting new gene 4 protein antibody
    • RING 4 antibody
    • RING4 antibody
    • TAP 1 antibody
    • TAP1 antibody
    • TAP1*0102N antibody
    • TAP1_HUMAN antibody
    • TAP1N antibody
    • Transporter 1 ATP binding cassette sub family B (MDR/TAP) antibody
    • Transporter 1 ATP binding cassette sub family B antibody
    • Transporter associated with antigen processing antibody
    • Transporter ATP binding cassette major histocompatibility complex 1 antibody
    • Y3 antibody
    see all

References

This product has been referenced in:
  • Zhang SY  et al. HMME-based PDT restores expression and function of transporter associated with antigen processing 1 (TAP1) and surface presentation of MHC class I antigen in human glioma. J Neurooncol 105:199-210 (2011). WB . Read more (PubMed: 21520005) »
  • Larrea E  et al. Oncostatin M enhances the antiviral effects of type I interferon and activates immunostimulatory functions in liver epithelial cells. J Virol 83:3298-311 (2009). WB ; Human . Read more (PubMed: 19158240) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Fibroblast cell line)
Loading amount
100 µg
Specification
Human Fibroblast cell line
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

Submitted Dec 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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