Overview

  • Product nameAnti-TAPA1 antibody [1D6]
    See all TAPA1 primary antibodies
  • Description
    Mouse monoclonal [1D6] to TAPA1
  • Specificityab23505 recognises human CD81, a 26kD cell surface antigen also known as TAPA-1, and a member of the tetraspanin family.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, IP, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human, Chimpanzee
  • Immunogen

    Aggregated OCI-LY8 cells (Human).

  • General notes


    This antibody induces homotypic adhesion and has powerful anti proliferative effects.

Properties

Applications

Our Abpromise guarantee covers the use of ab23505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionMay play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.
    • Tissue specificityHematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
    • Involvement in diseaseDefects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
    • Post-translational
      modifications
      Not glycosylated.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 26 kDa cell surface protein TAPA 1 antibody
      • 26 kDa cell surface protein TAPA-1 antibody
      • 26 kDa cell surface protein TAPA1 antibody
      • CD 81 antibody
      • CD81 antibody
      • CD81 antigen (target of antiproliferative antibody 1) antibody
      • CD81 antigen antibody
      • CD81 molecule antibody
      • CD81_HUMAN antibody
      • CVID6 antibody
      • S5.7 antibody
      • TAPA 1 antibody
      • TAPA1 antibody
      • Target of the antiproliferative antibody 1 antibody
      • Tetraspanin 28 antibody
      • Tetraspanin-28 antibody
      • Tetraspanin28 antibody
      • Tspan 28 antibody
      • Tspan-28 antibody
      • Tspan28 antibody
      see all

    References for Anti-TAPA1 antibody [1D6] (ab23505)

    This product has been referenced in:
    • Christianson HC  et al. Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity. Proc Natl Acad Sci U S A 110:17380-5 (2013). WB ; Human . Read more (PubMed: 24101524) »
    • Wang AL  et al. Autophagy and exosomes in the aged retinal pigment epithelium: possible relevance to drusen formation and age-related macular degeneration. PLoS One 4:e4160 (2009). Flow Cyt ; Human . Read more (PubMed: 19129916) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (gastric)
    Loading amount 20 µg
    Specification gastric
    Gel Running Conditions Non-reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted May 19 2009

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I am sorry but the expression of Tapa1 is outside of my area of expertese. I would appreciate it if you could explain to me why you are attempt...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"