Anti-CD81 antibody [1D6] (ab23505)
Key features and details
- Mouse monoclonal [1D6] to CD81
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD81 antibody [1D6]
See all CD81 primary antibodies -
Description
Mouse monoclonal [1D6] to CD81 -
Host species
Mouse -
Specificity
ab23505 recognises human CD81, a 26kD cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Goat, Chimpanzee -
Immunogen
Tissue, cells or virus corresponding to Human CD81. Aggregated OCI-LY8 cells (Human).
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Positive control
- Flow Cyt: HAP1-WT cells.
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General notes
This antibody induces homotypic adhesion and has powerful anti proliferative effects.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
This antibody induces homotypic adhesion and has powerful anti proliferative effects. -
Clonality
Monoclonal -
Clone number
1D6 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/5 - 1/10.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
1/5 - 1/10. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. -
Tissue specificity
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines. -
Involvement in disease
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsNot glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 449635 Chimpanzee
- Entrez Gene: 975 Human
- Omim: 186845 Human
- SwissProt: P60034 Chimpanzee
- SwissProt: P60033 Human
- Unigene: 54457 Human
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Alternative names
- 26 kDa cell surface protein TAPA 1 antibody
- 26 kDa cell surface protein TAPA-1 antibody
- 26 kDa cell surface protein TAPA1 antibody
see all
Images
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Overlay histogram showing HAP1 wildtype (green line) and HAP1-CD81 knockout cells (red line) stained with ab23505. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab23505, 0.1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.
A mouse IgG1 isotype control antibody (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CD81 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab23505 has been referenced in 13 publications.
- Krishn SR et al. Small extracellular vesicle-mediated ITGB6 siRNA delivery downregulates the αVβ6 integrin and inhibits adhesion and migration of recipient prostate cancer cells. Cancer Biol Ther 23:173-185 (2022). PubMed: 35188070
- Naranjo NM et al. IFIT3 (interferon induced protein with tetratricopeptide repeats 3) modulates STAT1 expression in small extracellular vesicles. Biochem J 478:3905-3921 (2021). PubMed: 34622927
- Sayeed A et al. Profiling the circulating mRNA transcriptome in human liver disease. Oncotarget 11:2216-2232 (2020). PubMed: 32577166
- Krishn SR et al. The avß6 integrin in cancer cell-derived small extracellular vesicles enhances angiogenesis. J Extracell Vesicles 9:1763594 (2020). PubMed: 32595914
- Quaglia F et al. Small extracellular vesicles modulated by aVß3 integrin induce neuroendocrine differentiation in recipient cancer cells. J Extracell Vesicles 9:1761072 (2020). PubMed: 32922691