Overview

  • Product nameAnti-TAPA1 antibody [EPR4244]
    See all TAPA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4244] to TAPA1
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TAPA1 aa 150-250.

  • Positive control
    • WB: Ramos, JAR, U87-MG, IM-9, peripheral blood mononuclear cell (PBMC), C6, PC-12, and RAW264.7 cell lysates.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab109201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.

For unpurified use at 1/500 - 1/1000.

  • Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • FunctionMay play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.
    • Tissue specificityHematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
    • Involvement in diseaseDefects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
    • Post-translational
      modifications
      Not glycosylated.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 26 kDa cell surface protein TAPA 1 antibody
      • 26 kDa cell surface protein TAPA-1 antibody
      • 26 kDa cell surface protein TAPA1 antibody
      • CD 81 antibody
      • Cd81 antibody
      • CD81 antigen (target of antiproliferative antibody 1) antibody
      • CD81 antigen antibody
      • CD81 molecule antibody
      • CD81_HUMAN antibody
      • CVID6 antibody
      • S5.7 antibody
      • TAPA 1 antibody
      • TAPA1 antibody
      • Target of the antiproliferative antibody 1 antibody
      • Tetraspanin 28 antibody
      • Tetraspanin-28 antibody
      • Tetraspanin28 antibody
      • Tspan 28 antibody
      • Tspan-28 antibody
      • Tspan28 antibody
      see all

    Anti-TAPA1 antibody [EPR4244] images

    • All lanes : Anti-TAPA1 antibody [EPR4244] (ab109201) at 1/700 dilution (unpurified)

      Lane 1 : Ramos cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : PC-12 cell lysate
      Lane 4 : Raw264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 26 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-TAPA1 antibody [EPR4244] (ab109201) at 1/2000 dilution (purified)

      Lane 1 : Ramos cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : PC-12 cell lysate
      Lane 4 : Raw264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 26 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-TAPA1 antibody [EPR4244] (ab109201) at 1/1000 dilution (unpurified)

      Lane 1 : Ramos cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : IM-9 cell lysate
      Lane 5 : Peripheral blood mononuclear cell (PBMC) cell lysate
      Lane 6 : C6 cell lysate
      Lane 7 : PC-12 cell lysate
      Lane 8 : RAW264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

      Predicted band size : 26 kDa

    References for Anti-TAPA1 antibody [EPR4244] (ab109201)

    This product has been referenced in:
    • Gu J  et al. Gastric cancer exosomes trigger differentiation of umbilical cord derived mesenchymal stem cells to carcinoma-associated fibroblasts through TGF-ß/Smad pathway. PLoS One 7:e52465 (2012). WB ; Human . Read more (PubMed: 23285052) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (Gradient Gel)
    Sample Hamster Cell lysate - whole cell (CHO cells)
    Specification CHO cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mrs. Soraia Martins

    Verified customer

    Submitted Mar 16 2015

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Non-Denaturing (Native) (12%)
    Sample Human Cell lysate - whole cell (Cancer cell)
    Specification Cancer cell
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Hyungtaek Jeon

    Verified customer

    Submitted Nov 06 2014

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Human Serum (Purified exosomes from human serum)
    Specification Purified exosomes from human serum
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 20 2014

    Application Western blot
    Loading amount 1e+006 cells
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
    Specification mouse embryonic stem cell
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 21 2014

    Thank you for your reply and protocol information.
    If we cannot get this antibody to work as stated on the datasheet we will replace it with a new vial of the same antibody or a different antibody of your choosing free of charge as indicated in our...

    Read More

    Thank you for contacting Abcam.
    I am sorry to hear that this antibody is not working for you. As this was a free antibody given through our RabMab promotion, we cannot issue a refund in this case. However, if we cannot work with you to get ab109201...

    Read More

    Thank you for your participation in Abcam's post complaint survey.

    Please let me know if there is anything else I can do for you.

    Also, if you have further questions to any of our products (e.g. primary antibodies, lysates, secondar...

    Read More

    Vielen Dank für Ihre Antwort.

    Es tut mir leid, dass Sie Probleme mit diesen beiden Antikörpern hatten. Wie per Emailbesprochen, habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer xx...

    Read More

    Vielen Dank fuer Ihre email.

    Ich habe nach Sekundaerantikoerpern in unserem Katalog geschaut. Wir haben zahlreiche, aber die folgenden haben wir momentan im Lager:

    ab16284 donkey anti-rabbit, HRP(http://www.abcam.com/Rabbit-IgG-seco...

    Read More

    Vielen Dank fuer Ihre email.

    In diesem Falle koennte ich Ihnen auch einen Ersatz fuer den Primaerantikoerper anbieten.

    Wuerden Sie einen anderen C81 Antikoerper probieren wollen, da wir kein neues Batch fuer ab109201 in Lager haben?...

    Read More

    1-10 of 15 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"