MOLT4 (human T-ALL cell line)
Our Abpromise guarantee covers the use of ab79559 in the following tested applications.
|WB||Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 26 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration. In human MOLT4 T cell line ab79559 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV 1)-positive human T-cell lines.|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
IHC image of TAPA1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79559, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.