Overview

  • Product name
  • Description
    Rabbit polyclonal to Tapasin
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    LGWAAVYLSTCKDSKKKAE

    , corresponding to amino acids 430-448 of Human Tapasin.

  • Positive control
    • This antibody gave a positive signal in A431 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/100.

Target

  • Function
    Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
  • Tissue specificity
    Neutrophils, mostly in fully differentiated cells.
  • Sequence similarities
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Domain
    The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • NGS 17 antibody
    • NGS-17 antibody
    • NGS17 antibody
    • TAP associated protein antibody
    • TAP binding protein antibody
    • TAP binding protein (tapasin) antibody
    • TAP BP antibody
    • TAP-associated protein antibody
    • TAP-binding protein antibody
    • TAPA antibody
    • Tapasin antibody
    • TAPBP antibody
    • TPN antibody
    • TPSN antibody
    • TPSN_HUMAN antibody
    see all

Images

  • Anti-Tapasin antibody (ab13518) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa


    Exposure time : 12 minutes

References

This product has been referenced in:
  • Del Campo AB  et al. Adenovirus expressing ß2-microglobulin recovers HLA class I expression and antitumor immunity by increasing T-cell recognition. Cancer Gene Ther N/A:N/A (2014). Read more (PubMed: 24971583) »
  • Chapiro J  et al. Destructive cleavage of antigenic peptides either by the immunoproteasome or by the standard proteasome results in differential antigen presentation. J Immunol 176:1053-61 (2006). WB ; Human . Read more (PubMed: 16393993) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Fibroblast cell line)
Loading amount
100 µg
Specification
Human Fibroblast cell line
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Dec 18 2012

I'm sorry to hear your customer is having problems with ab13518 and thank him for taking the time to give us protocol details. We have not received any complaints regarding this antibody and are confused by the complaint form: the customer mentions...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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