Overview

  • Product name
  • Description
    Rabbit polyclonal to Tapasin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    LGWAAVYLSTCKDSKKKAE

    , corresponding to amino acids 430-448 of Human Tapasin.

  • Positive control
    • This antibody gave a positive signal in A431 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/100.

Target

  • Function
    Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
  • Tissue specificity
    Neutrophils, mostly in fully differentiated cells.
  • Sequence similarities
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Domain
    The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • NGS 17 antibody
    • NGS-17 antibody
    • NGS17 antibody
    • TAP associated protein antibody
    • TAP binding protein antibody
    • TAP binding protein (tapasin) antibody
    • TAP BP antibody
    • TAP-associated protein antibody
    • TAP-binding protein antibody
    • TAPA antibody
    • Tapasin antibody
    • TAPBP antibody
    • TPN antibody
    • TPSN antibody
    • TPSN_HUMAN antibody
    see all

Images

  • Anti-Tapasin antibody (ab13518) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa


    Exposure time: 12 minutes

References

This product has been referenced in:
  • Del Campo AB  et al. Adenovirus expressing ß2-microglobulin recovers HLA class I expression and antitumor immunity by increasing T-cell recognition. Cancer Gene Ther N/A:N/A (2014). Read more (PubMed: 24971583) »
  • Chapiro J  et al. Destructive cleavage of antigenic peptides either by the immunoproteasome or by the standard proteasome results in differential antigen presentation. J Immunol 176:1053-61 (2006). WB ; Human . Read more (PubMed: 16393993) »

See all 2 Publications for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Fibroblast cell line)
Loading amount
100 µg
Specification
Human Fibroblast cell line
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

Submitted Dec 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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