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Rabbit polyclonal to Tapasin
Predicted to work with:
, corresponding to amino acids 430-448 of Human Tapasin.
This antibody gave a positive signal in A431 whole cell lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: Whole Serum
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
Neutrophils, mostly in fully differentiated cells.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region.
Endoplasmic reticulum membrane.
Information by UniProt
NGS 17 antibody
Western blot - Anti-Tapasin antibody (ab13518)
Anti-Tapasin antibody (ab13518) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
This product has been referenced in:
Del Campo AB
et al. Adenovirus expressing ß2-microglobulin recovers HLA class I expression and antitumor immunity by increasing T-cell recognition. Cancer Gene Ther N/A:N/A (2014).
Read more (PubMed: 24971583) »
et al. Destructive cleavage of antigenic peptides either by the immunoproteasome or by the standard proteasome results in differential antigen presentation. J Immunol 176:1053-61 (2006).
Read more (PubMed: 16393993) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"