Overview

  • Product nameAnti-Tapasin Related Protein antibody
    See all Tapasin Related Protein primary antibodies
  • Description
    Mouse monoclonal to Tapasin Related Protein
  • Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: PHPAEGQWRA VDVVLDCFLV KDGAHRGALA SSEDRARASL VLKQVPVLDD GSLEDFTDFQ GGTLAQDDPP IIFEASVDLV QIPQAEALLH ADCSGKEVT, corresponding to amino acids 23-122 of Human Tapasin Related Protein

Properties

Applications

Our Abpromise guarantee covers the use of ab57411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 50 kDa.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration. PubMed: 23401559

Target

  • Sequence similaritiesContains 1 Ig-like C1-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Microsome membrane. Mainly found in endoplasmic reticulum but a minority is found on the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10143 antibody
    • TAP binding protein like antibody
    • TAP binding protein related antibody
    • TAP binding protein related protein antibody
    • TAP-binding protein-like antibody
    • TAP-binding protein-related protein antibody
    • Tapasin like antibody
    • Tapasin R antibody
    • Tapasin-like antibody
    • TAPASIN-R antibody
    • Tapasin-related protein antibody
    • TAPBP L antibody
    • TAPBP R antibody
    • TAPBP-R antibody
    • Tapbpl antibody
    • TAPBPR antibody
    • TPSNR_HUMAN antibody
    see all

Anti-Tapasin Related Protein antibody images



  • Predicted band size : 50 kDa
    Tapasin Related Protein antibody (ab57411) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • Overlay histogram showing A431 cells stained with ab57411 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57411, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Tapasin Related Protein antibody (ab57411)

This product has been referenced in:
  • Boyle LH  et al. Tapasin-related protein TAPBPR is an additional component of the MHC class I presentation pathway. Proc Natl Acad Sci U S A 110:3465-70 (2013). WB ; Human . Read more (PubMed: 23401559) »
  • Hermann C  et al. The binding of TAPBPR and Tapasin to MHC class I is mutually exclusive. J Immunol 191:5743-50 (2013). IP . Read more (PubMed: 24163410) »

See all 2 Publications for this product

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