Our Abpromise guarantee covers the use of ab109836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa. The detection limit for recombinant Human TARC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Neutralising||Use a concentration of 2 - 3 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of Human TARC (100 ng/ml)|
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. ab109836 in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TARC (using 100 µl/well antibody solution).|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to TARC (Biotin) (ab84279). ab109836 in conjunction with ab84279 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TARC (using 100 µl/well antibody solution).|