Overview

  • Product name
    Anti-TARS antibody [TARSF8H3]
    See all TARS primary antibodies
  • Description
    Mouse monoclonal [TARSF8H3] to TARS
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of human TARS

  • Positive control
    • WB: HeLa whole cell lysate ICC/IF: HeLa cell

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. It is sterile filtered through a 0.22 µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    TARSF8H3
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).
ICC 1/5.

Target

  • Sequence similarities
    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic antibody
    • MGC9344 antibody
    • SYTC_HUMAN antibody
    • TARS antibody
    • Threonine tRNA ligase 1 antibody
    • Threonine tRNA ligase 1, cytoplasmic antibody
    • Threonine--tRNA ligase antibody
    • Threonyl tRNA synthetase antibody
    • Threonyl tRNA synthetase, cytoplasmic antibody
    • Threonyl-tRNA synthetase antibody
    • ThrRS antibody
    see all

Images

  • Anti-TARS antibody [TARSF8H3] (ab50795) at 1/50 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 83 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Ab50795, at a dilution of 1/5, staining TARS in HeLa cell by Immunocytochemistry/Immunofluorescence. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/200.

References

ab50795 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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