![Western blot - TARS antibody [TARSF8H3] (ab50795) image](#ab50795_1.JPG)
![Immunocytochemistry/ Immunofluorescence - TARS antibody [TARSF8H3] (ab50795) image](#ab50795_2.JPG)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Overview
Product name
Anti-TARS antibody [TARSF8H3]
See all TARS products (3) ...
Description
Mouse monoclonal [TARSF8H3] to TARS
Tested applications
WB, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment of human TARS
Positive control
WB: HeLa whole cell lysate ICC/IF: HeLa cell
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. It is sterile filtered through a 0.22 µm membrane.
Clonality
Monoclonal
Clone number
TARSF8H3
Isotype
IgG2a
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).
ICC
ICC: 1/5ICC: 1/5
Target
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP26639
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytoplasmic antibody
- MGC9344 antibody
- SYTC_HUMAN antibody
see all
Database links
- Entrez Gene: 6897 Human
- Omim: 187790 Human
- SwissProt: P26639 Human
- Unigene: 481860 Human
Anti-TARS antibody [TARSF8H3] images:
Western blot - TARS antibody [TARSF8H3] (ab50795)
![Western blot - TARS antibody [TARSF8H3] (ab50795)](/ps/datasheet/Images/50/ab50795/ab50795_1.JPG)
Anti-TARS antibody [TARSF8H3] (ab50795) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size : 83 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - TARS antibody [TARSF8H3] (ab50795)
![Immunocytochemistry/ Immunofluorescence - TARS antibody [TARSF8H3] (ab50795)](/ps/datasheet/Images/50/ab50795/ab50795_2.JPG)
Ab50795, at a dilution of 1/5, staining TARS in HeLa cell by Immunocytochemistry/Immunofluorescence. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/200.
References for Anti-TARS antibody [TARSF8H3] (ab50795)
ab50795 has not yet been referenced specifically in any publications.
Publishing research using ab50795? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"