Anti-TATA binding protein TBP antibody [58C9] (ab61411)

Overview

  • Product nameAnti-TATA binding protein TBP antibody [58C9]
    See all TATA binding protein TBP primary antibodies
  • Description
    Mouse monoclonal [58C9] to TATA binding protein TBP
  • Specificityab61411 recognises TATA binding protein TBP also in Sf9 cells.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human, Saccharomyces cerevisiae, Drosophila melanogaster, Corn
  • Immunogen

    Drosophila TFIID complex.

  • Positive control
    • Nuclear extracts of D melanogaster cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab61411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa).
IP Use at an assay dependent dilution.

Target

  • FunctionGeneral transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
  • Tissue specificityWidely expressed, with levels highest in the testis and ovary.
  • Involvement in diseaseDefects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
  • Sequence similaritiesBelongs to the TBP family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • GTF2D antibody
      • GTF2D1 antibody
      • HDL4 antibody
      • MGC117320 antibody
      • MGC126054 antibody
      • MGC126055 antibody
      • SCA17 antibody
      • TATA binding factor antibody
      • TATA box factor antibody
      • TATA sequence binding protein antibody
      • TATA sequence-binding protein antibody
      • TATA-binding factor antibody
      • TATA-box binding protein N-terminal domain antibody
      • TATA-box factor antibody
      • TATA-box-binding protein antibody
      • TBP antibody
      • TBP_HUMAN antibody
      • TFIID antibody
      • Transcription initiation factor TFIID TBP subunit antibody
      see all

    Anti-TATA binding protein TBP antibody [58C9] images

    • Lane 1 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 2 µg/ml
      Lane 2 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 1 µg/ml
      Lane 3 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 0.5 µg/ml
      Lane 4 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 0.2 µg/ml
      Lane 5 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) (Negative control )

      Lane 1 : Nuclear cell extract of D.Mel cells
      Lane 2 : Nuclear cell extract of D.Mel cells
      Lane 3 : Nuclear cell extract of D.Mel cells
      Lane 4 : Nuclear cell extract of D.Mel cells
      Lane 5 : Nuclear cell extract of D.Mel cells

      Lysates/proteins at 20 µg/ml per lane.

      Secondary
      Goat Anti-Mouse – Peroxidase conjugate
      Developed using the ECL technique

      Predicted band size : 38 kDa
      Observed band size : 39 kDa (why is the actual band size different from the predicted?)

    References for Anti-TATA binding protein TBP antibody [58C9] (ab61411)

    This product has been referenced in:
    • Li L  et al. A specific E3 ligase/deubiquitinase pair modulates TBP protein levels during muscle differentiation. Elife 4:e08536 (2015). Read more (PubMed: 26393420) »
    • von Marschall Z & Fisher LW Secreted Frizzled-related protein-2 (sFRP2) augments canonical Wnt3a-induced signaling. Biochem Biophys Res Commun 400:299-304 (2010). WB ; Human . Read more (PubMed: 20723538) »

    See all 3 Publications for this product

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    I am sorry to confirm that our source has not determined whether this particular antibody is kappa or light chain.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"