Anti-TATA Element Modulatory Factor 1 antibody (ab121290)

Overview

  • Product name
    Anti-TATA Element Modulatory Factor 1 antibody
    See all TATA Element Modulatory Factor 1 primary antibodies
  • Description
    Rabbit polyclonal to TATA Element Modulatory Factor 1
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 253-397 of Human TATA Element Modulatory Factor 1.

  • Positive control
    • Human placenta tissue; U 2 OS cells; RT 4 and U 251 MG cell lysates.

Applications

Our Abpromise guarantee covers the use of ab121290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/250 - 1/500.

Target

  • Function
    Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP).
  • Domain
    The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • Cellular localization
    Cytoplasm. Nucleus. Golgi apparatus membrane. Concentrated at the budding structures localized at the tips of cisternae.
  • Information by UniProt
  • Database links
  • Alternative names
    • Androgen receptor coactivator 160 kDa protein antibody
    • Androgen receptor-associated protein of 160 kDa antibody
    • ARA160 antibody
    • TATA element modulatory factor 1 antibody
    • TATA element modulatory factor antibody
    • TMF antibody
    • Tmf1 antibody
    • TMF1_HUMAN antibody
    see all

Images

  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • ab121290, at a 1/200 dilution, staining TATA Element Modulatory Factor 1 in trophoblastic cells of paraffin-embedded Human placenta tissue by immunohistochemistry.
  • ab121290, at 4 µg/ml, staining TATA Element Modulatory Factor 1 in PFA/Triton X-100 treated Human U 2 OS cells by Immunofluorescence (green).
  • All lanes : Anti-TATA Element Modulatory Factor 1 antibody (ab121290) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

ab121290 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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