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Other Immunogen Type corresponding to Cow Tau. Purified bovine microtubule-associated proteins.
Our Abpromise guarantee covers the use of ab3931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. PubMed: 22699908|
|IHC-Fr||1/25 - 1/50. We suggest an incubation period of 30 minutes at room temperature.|
|WB||1/50 - 1/100. Predicted molecular weight: 60 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
Immunofluorescence analysis of Human primary culture neurons, either untreated (left) or treated with 500 nM quinolinic acid (right).
Tau (red) was stained using ab3931. An AlexaFluor®594-conjugated goat anti-mouse IgG (ab150116) was used as the secondary antibody. Nuclei were stained with DAPI (blue).
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