Recombinant
RabMAb

Anti-TAX1BP3 antibody [EPR7900(2)] (ab155992)

Overview

  • Product name
    Anti-TAX1BP3 antibody [EPR7900(2)]
    See all TAX1BP3 primary antibodies
  • Description
    Rabbit monoclonal [EPR7900(2)] to TAX1BP3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human TAX1BP3 (Uniprot O14907).

  • Positive control
    • Human fetal heart, NIH/3T3, Rat heart, A673 and HT-1080 lysates; permeabilized A673 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      May play a role in the Rho signaling pathway. May act as an inhibitor of the Wnt signaling pathway. May play a role in activation of CDC42 by the viral protein HPV16 E6.
    • Tissue specificity
      Detected in various cell lines including HeLa. Weakly expressed in peripheral blood leukocytes.
    • Sequence similarities
      Contains 1 PDZ (DHR) domain.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Glutaminase interacting protein 3 antibody
      • Glutaminase-interacting protein 3 antibody
      • Tax interaction protein 1 antibody
      • Tax1 (human T cell leukemia virus type I) binding protein 3 antibody
      • Tax1 binding protein 3 antibody
      • Tax1-binding protein 3 antibody
      • Tax1bp3 antibody
      • TIP 1 antibody
      • TIP-1 antibody
      • TIP1 antibody
      • TX1B3_HUMAN antibody
      see all

    Images

    • All lanes : Anti-TAX1BP3 antibody [EPR7900(2)] (ab155992) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : NIH/3T3 lysate
      Lane 3 : Rat heart lysate
      Lane 4 : A673 lysate
      Lane 5 : HT-1080 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 14 kDa

    • Flow Cytometric analysis of permeabilized A673 cells labeling TAX1BP3 with ab155992 at 1/100 dilution (red) compared with a rabbit IgG negative control (green).

    References

    ab155992 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Embryonic Heart)
    Permeabilization
    Yes - Tween-20
    Specification
    Embryonic Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 11 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 06 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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