The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/10000.
Antigen is coated on EIA strips at 0.4 µg per well. Antiserum is diluted in series from 1/10 to 1/10,000 and added in separate wells.
Incubate the antibody for 0.5 hr. Wash off unbound antibodies and add anti-rabbit IgG-HRP conjugate. Wash the plates and add substrate to develop color for 5 min. Read absorbance at 405 nm. The intensity of the colour is directly proportional to the amount of antigen present.
A positive is when the sample signal is double that of the control or pre-immune serum.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceTaxol (paclitaxel) is particularly effective as an anticancer agent because tubulin is not its only target. It also binds to a protein called Bcl2, which normally blocks the process of apoptosis, or cell death. Taxol therefore plays a dual role in destroying dividing cells,firstly by stabilising assembled microtubules and thereby halting mitosis, and secondly by inhibiting Bcl2 and allowing apoptosis to proceed.
References for Anti-Taxol antibody (ab26953)
has not yet been referenced specifically in any publications.
Publishing research using ab26953? Please let us know so that we can cite the reference in this datasheet.
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