Overview

  • Product nameAnti-TAZ antibody
    See all TAZ primary antibodies
  • Description
    Mouse monoclonal to TAZ
  • Tested applicationsSuitable for: WB, IHC-P, Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 201-300 of Human TAZ with a proprietary tag (NP_056287).

  • Positive control
    • Human placenta tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab118373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 44 kDa.
IHC-P Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.
Sandwich ELISA Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • FunctionTranscriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
  • Tissue specificityHighly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
  • Sequence similaritiesContains 1 WW domain.
  • DomainThe PDZ-binding motif is essential for stimulated gene transcription. It localizes the protein into both punctate nuclear foci and plasma membrane-associated complexes.
    Binds to transcription factors via its WW domain.
  • Post-translational
    modifications
    Phosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export.
  • Cellular localizationNucleus. Cytoplasm. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated, is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP586I1419 antibody
    • FLJ27004 antibody
    • FLJ45718 antibody
    • OTTHUMP00000215994 antibody
    • OTTHUMP00000215995 antibody
    • OTTHUMP00000215996 antibody
    • OTTHUMP00000216001 antibody
    • TAZ antibody
    • Transcriptional co activator with PDZ binding motif antibody
    • Transcriptional coactivator with PDZ binding motif antibody
    • Transcriptional coactivator with PDZ-binding motif antibody
    • WW domain containing transcription regulator 1 antibody
    • WW domain containing transcription regulator protein 1 antibody
    • WW domain-containing transcription regulator protein 1 antibody
    • WWTR 1 antibody
    • WWTR1 antibody
    • WWTR1_HUMAN antibody
    see all

Anti-TAZ antibody images

  • Anti-TAZ antibody (ab118373) at 5 µg/ml + Recombinant immunogen at 0.2 µg

    Predicted band size : 44 kDa
  • ab118272 at 3µg/ml staining TAZ in Formalin-fixed, Paraffin-embedded Human placenta tissue by Immunohistochemistry.
  • Detection limit for recombinant tagged TAZ is 0.3 ng/ml as a capture antibody.

References for Anti-TAZ antibody (ab118373)

This product has been referenced in:
  • Cardoso J  et al. CYR61 and TAZ Upregulation and Focal Epithelial to Mesenchymal Transition May Be Early Predictors of Barrett's Esophagus Malignant Progression. PLoS One 11:e0161967 (2016). IHC-P ; Human . Read more (PubMed: 27583562) »
  • Wang L  et al. Overexpression of YAP and TAZ is an independent predictor of prognosis in colorectal cancer and related to the proliferation and metastasis of colon cancer cells. PLoS One 8:e65539 (2013). WB, IHC-P ; Human . Read more (PubMed: 23762387) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry regarding ab118373 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

After reading through the detailed protocol you ...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
Loading amount 50000 cells
Specification MDA-MB-231 breast cancer cell line
Gel Running Conditions Reduced Denaturing (10% PAGE)
Blocking step 2% milk + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 21 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

Thank you for contacting us.

I have compared the sequences of the TAZ with the sequenz of the YAP1 protein, and it looks like this two proteins are two 49% identical and to ˜70% similar. However, I found that all our antibodies are derived f...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vita...

Read More

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

Read More

Merci de nous avoir contactés.

Les anti-TAZ ab119253 et ab118373 sont tous les 2 des monoclonaux de souris qui ont été testés sur échantillons humains et en Western Blot.
http://www.abcam.com/ab118373
Read More

Thank you for contacting us about this discrepancy. You are correct, the IHC image on the ab118272 datasheetshows staining of human placenta, not pancreas. I apologize for the error on our datasheet, and I will have this corrected as soon as possible. ...

Read More

Thank you for your enquiry and your interest in our products.   I have conducted a search for you and for your customer and 4 antibodies come up under TAZ target name using the advanced search engine for primary antibodies:   -  ab118373, -...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"