The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
TBC1D10B, which belongs to the TBC1 domain family, member 10B, was experimentally validated as a Hela cell nuclei protein, currently with unknown function . TBC/rabGAP domain-containing microvillar protein binds to first PDZ domains of EBP50 and E3KARP and EBP50-PDZ and may be potentially involved in regulating membrane traffic.