Overview

  • Product name
  • Description
    Rabbit polyclonal to TBC1D13
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TBC1D13 aa 83-113 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9NVG8

  • Positive control
    • CEM cell line lysate and Mouse spleen tissue lysate; Mouse heart tissue; NCI-H460 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 47 kDa.

Target

  • Relevance
    TBC1D13 may act as a GTPase-activating protein for Rab family protein(s). It contains 1 Rab-GAP TBC domain.
  • Cellular localization
    Intracellular
  • Database links
  • Alternative names
    • 2600014A06Rik antibody
    • BC025586 antibody
    • FLJ10743 antibody
    • MGC38260 antibody
    • OTTHUMP00000022298 antibody
    • OTTMUSP00000018144 antibody
    • RP11 545E17.5 antibody
    • RP23 315H12.1 antibody
    • TBC1 domain family member 13 antibody
    see all

Images

  • Anti-TBC1D13 antibody (ab170529) at 1/100 dilution + CEM cell line lysates at 35 µg

    Developed using the ECL technique.

    Predicted band size: 47 kDa

  • Anti-TBC1D13 antibody (ab170529) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 47 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse heart tissue labeling TBC1D13 with ab170529 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Immunofluorescence analysis of NCI-H460 cells labeling TBC1D13 with ab170529 at 1/10 dilution, followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

References

ab170529 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170529.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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