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- Product nameAnti-TBC1D15 antibody
See all TBC1D15 primary antibodies
Rabbit polyclonal to TBC1D15
- Tested applicationsSuitable for:
ICC/IF, IHC-P, WBmore details
- Species reactivity
Mouse, Rat, Human
antigen sequence, corresponding to internal sequence amino acids 169-286 of Human TBC1D15, isoform 2.
- Positive control
- Human testis tissue. RT 4 and U 251 MG cell lysates; Human liver and tonsil tissue lysates.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
- PurityImmunogen affinity purified
- Research areas
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
- FunctionActs as a GTPase activating protein for RAB7A. Does not act on RAB4, RAB5 or RAB6.
- Sequence similaritiesContains 1 Rab-GAP TBC domain.
- Cellular localizationCytoplasm.
Information by UniProt
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Anti-TBC1D15 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-TBC1D15 antibody (ab121396)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and mitochondria. Recommended concentration of ab121396 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab121396)
ab121396 at 1/40 dilution staining TBC1D15 in paraffin-embedded Human testis tissue by Immunohistochemistry.
Western blot - Anti-TBC1D15 antibody (ab121396)
All lanes : Anti-TBC1D15 antibody (ab121396) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique
References for Anti-TBC1D15 antibody (ab121396)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"