Overview

  • Product nameAnti-TBC1D4 antibody [5E6]
    See all TBC1D4 primary antibodies
  • Description
    Mouse monoclonal [5E6] to TBC1D4
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human TBC1D4 protein produced in HEK293T cells (NP_055647.2).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY TBC1D4 cDNA; COS7 cells transiently transfected with pCMV6-ENTRY TBC1D4; HEK293T cells transfected with pCMV6-ENTRY TBC1D4 overexpress plasmid; HeLa and Jurkat cells.
  • General notesDilute in PBS (pH 7.3) before use. Stable for 12 months from date of receipt.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, 48% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab119004 is purified from Mouse ascites fluid by affinity chromatography.
  • ClonalityMonoclonal
  • Clone number5E6
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 147 kDa.
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionMay act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
  • Tissue specificityWidely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.
  • Sequence similaritiesContains 2 PID domains.
    Contains 1 Rab-GAP TBC domain.
  • Post-translational
    modifications
    Phosphorylated by AKT1; insulin-induced.
    Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
    Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
  • Cellular localizationCytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acrg embryonic lethality (mouse) minimal region ortholog antibody
    • Acrg embryonic lethality minimal region ortholog antibody
    • Acrg embryonic lethality mouse minimal region ortholog antibody
    • Akt substrate of 160 kDa antibody
    • AS 160 antibody
    • AS160 antibody
    • BUB2 antibody
    • CDC16 antibody
    • KIAA0603 antibody
    • TBC (Tre 2 BUB2 CDC16) domain containing protein antibody
    • TBC Tre 2 BUB2 CDC16 domain containing protein antibody
    • TBC1 D4 antibody
    • TBC1 domain family member 4 antibody
    • Tbc1d4 antibody
    • TBCD4_HUMAN antibody
    • Tre-2 antibody
    see all

Anti-TBC1D4 antibody [5E6] images

  • All lanes : Anti-TBC1D4 antibody [5E6] (ab119004) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs and lysed
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY TBC1D4 cDNA for 48 hrs and lysed

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 147 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202079)
  • ab119004, at a 1/100 dilution, staining TBC1D4 in COS7 cells transiently transfected with pCMV6-ENTRY TBC1D4, by Immunofluorescence.
  • ab119004 at 1/100 dilution staining TBC1D4 in HEK293T cells transfected with either pCMV6-ENTRY TBC1D4 overexpress plasmid (Red) or empty vector control plasmid(Blue) by Flow Cytometry.
  • ab119004 at 1/100 dilution staining TBC1D4 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab119004 at 1/100 dilution staining TBC1D4 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References for Anti-TBC1D4 antibody [5E6] (ab119004)

ab119004 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"