The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 147 kDa).
Use at an assay dependent concentration. PubMed: 20937822
1/135. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionMay act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Tissue specificityWidely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.
Post-translational modificationsPhosphorylated by AKT1; insulin-induced. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
Cellular localizationCytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
Acrg embryonic lethality (mouse) minimal region ortholog antibody
Acrg embryonic lethality minimal region ortholog antibody
Acrg embryonic lethality mouse minimal region ortholog antibody
Akt substrate of 160 kDa antibody
AS 160 antibody
TBC (Tre 2 BUB2 CDC16) domain containing protein antibody
TBC Tre 2 BUB2 CDC16 domain containing protein antibody
TBC1 D4 antibody
TBC1 domain family member 4 antibody
Anti-TBC1D4 antibody images
Western blot - Anti-TBC1D4 antibody (ab24469)This image is courtesy of Jonas Treebak
All lanes : Anti-TBC1D4 antibody (ab24469) at 0.46 µg/ml
Lane 1 : Human skeletal muscle cell lysate at 10 µg Lane 2 : Human skeletal muscle cell lysate at 20 µg Lane 3 : Human skeletal muscle cell lysate at 40 µg Lane 4 : Human skeletal muscle cell lysate at 60 µg
ab24469 staining TBC1D4 in human stomach. Paraffin embedded human stomach tissue was incubated with ab24469 (1/135 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab24469 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Kim TY et al. Substrate trapping proteomics reveals targets of the ßTrCP2/FBXW11 ubiquitin ligase. Mol Cell Biol35:167-81 (2015).
Read more (PubMed: 25332235) »
Treebak JT et al. Acute exercise and physiological insulin induce distinct phosphorylation signatures on TBC1D1 and TBC1D4 proteins in human skeletal muscle. J Physiol592:351-75 (2014).
Read more (PubMed: 24247980) »