Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab59121 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 147 kDa (predicted molecular weight: 147 kDa).
    Peptide ELISA: antibody detection limit dilution 1/128000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
    • Tissue specificity
      Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.
    • Sequence similarities
      Contains 2 PID domains.
      Contains 1 Rab-GAP TBC domain.
    • Post-translational
      modifications
      Phosphorylated by AKT1; insulin-induced.
      Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
      Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
    • Cellular localization
      Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acrg embryonic lethality (mouse) minimal region ortholog antibody
      • Acrg embryonic lethality minimal region ortholog antibody
      • Acrg embryonic lethality mouse minimal region ortholog antibody
      • Akt substrate of 160 kDa antibody
      • AS 160 antibody
      • AS160 antibody
      • BUB2 antibody
      • CDC16 antibody
      • KIAA0603 antibody
      • NIDDM5 antibody
      • TBC (Tre 2 BUB2 CDC16) domain containing protein antibody
      • TBC Tre 2 BUB2 CDC16 domain containing protein antibody
      • TBC1 D4 antibody
      • TBC1 domain family member 4 antibody
      • Tbc1d4 antibody
      • TBCD4_HUMAN antibody
      • Tre-2 antibody
      see all

    Images

    • ab59121 staining TBC1D4 in human skeletal muscle tissue lysate at 3.8ug/mL. Antigen retrieval was with a citrate buffer pH 6.

       

    • Anti-TBC1D4 antibody (ab59121) at 0.1 µg/ml (incubated for 1 hour) + Daudi cell lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 147 kDa
      Observed band size : 147 kDa

    References

    This product has been referenced in:
    • Berenguer M  et al. A serum factor induces insulin-independent translocation of GLUT4 to the cell surface which is maintained in insulin resistance. PLoS One 5:e15560 (2010). WB ; Human . Read more (PubMed: 21187969) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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