Overview

  • Product name
  • Description
    Rabbit polyclonal to TBC1D9B
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: RKASVVDPST ESSPAPQEGS EQPASPASPL SSRQSFCAQE APTASQGLLK LFQKNSPMED LGAKGAKEKM KEESWHIHFF EYG, corresponding to amino acids 408-490 of Human TBC1D9B.

  • Positive control
    • Human pancreas tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May act as a GTPase-activating protein for Rab family protein(s).
  • Sequence similarities
    Contains 1 EF-hand domain.
    Contains 2 GRAM domains.
    Contains 1 Rab-GAP TBC domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0676 antibody
    • TBC1 domain family member 9B antibody
    • TBC1 domain family, member 9B (with GRAM domain) antibody
    • TBC1D9B antibody
    • TBC9B_HUMAN antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and plasma membrane. Recommended concentration of ab122462 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122462, at 1/200 dilution, staining TBC1D9B in paraffin-embedded Human pancreas tissue by Immunohistochemistry.

References

ab122462 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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