Overview

  • Product nameAnti-TBL1 antibody
    See all TBL1 primary antibodies
  • Description
    Goat polyclonal to TBL1
  • SpecificityThis antibody is also expected to recognise the human protein TBLR1 (NP_078941), which is very similar.
  • Tested applicationsSuitable for: IHC, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human TBL1 aa 564-577 (C terminal).
    Sequence:

    SASDGSVCVLDLRK


    Database link: O60907
    (Peptide available as ab22881)

  • General notesPrincipal Names - TBL1X; transducin (beta)-like 1X-linked; EBI; TBL1; transducin (beta)-like 1; transducin (beta)-like 1 X-linked. Official Gene Symbol - TBL1X. GenBank Accession Number – NP_005638. LocusLink ID - 6907.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use a concentration of 4 - 6 µg/ml.
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:4,000.

WB Use a concentration of 3 - 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human TBL1 peptide (ab22881).

Target

  • FunctionF-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the WD repeat EBI family.
    Contains 1 F-box-like domain.
    Contains 1 LisH domain.
    Contains 8 WD repeats.
  • DomainThe F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EBI antibody
    • F box like/WD repeat protein TBL1X antibody
    • F-box-like/WD repeat-containing protein TBL1X antibody
    • SMAP 55 antibody
    • SMAP55 antibody
    • TBL 1 antibody
    • TBL 1X antibody
    • TBL1 antibody
    • TBL1X antibody
    • TBL1X_HUMAN antibody
    • Transducin (beta) like 1 antibody
    • Transducin (beta) like 1 X linked antibody
    • Transducin (beta) like 1X linked antibody
    • Transducin beta like 1 X linked antibody
    • Transducin beta like 1X antibody
    • Transducin beta like 1X protein antibody
    • Transducin beta like protein 1, X linked antibody
    • Transducin beta-like protein 1X antibody
    • Transducin-beta-like protein 1 antibody
    • X-linked antibody
    see all

Anti-TBL1 antibody images

  • Immunohistochemical analysis of paraffin embedded Human Kidney tissue labeling TBL1 with ab5642 at 4 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.



  • Predicted band size : 58 kDa
    ab2243 staining (4µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab2243 staining (4µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References for Anti-TBL1 antibody (ab2243)

This product has been referenced in:
  • Hoberg JE  et al. SMRT derepression by the IkappaB kinase alpha: a prerequisite to NF-kappaB transcription and survival. Mol Cell 16:245-55 (2004). Read more (PubMed: 15494311) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"