Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
Key features and details
- Mouse monoclonal [4F3-A8-D9] to TBLR1/TBL1XR1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9]
See all TBLR1/TBL1XR1 primary antibodies -
Description
Mouse monoclonal [4F3-A8-D9] to TBLR1/TBL1XR1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey -
Immunogen
Recombinant fragment corresponding to Human TBLR1/TBL1XR1.
Database link: Q9BZK7 -
Positive control
- WB: K562 whole cell lysate, Mouse heart lysate, Mouse brain lysate ICC: HeLa cells IHC-P: Human breast cancer
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General notes
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Concentrated Culture Supernatant -
Clonality
Monoclonal -
Clone number
4F3-A8-D9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
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IHC-P |
1/500 - 1/1000.
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ICC/IF |
1/150 - 1/200.
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Notes |
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WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa). |
IHC-P
1/500 - 1/1000. |
ICC/IF
1/150 - 1/200. |
Target
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Function
F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the WD repeat EBI family.
Contains 1 F-box-like domain.
Contains 1 LisH domain.
Contains 8 WD repeats. -
Domain
The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79718 Human
- Entrez Gene: 81004 Mouse
- Omim: 608628 Human
- SwissProt: Q9BZK7 Human
- SwissProt: Q8BHJ5 Mouse
- Unigene: 581171 Human
- Unigene: 202966 Mouse
- Unigene: 446265 Mouse
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Alternative names
- C21 antibody
- DC42 antibody
- F box like/WD repeat containing protein TBL1XR1 antibody
see all
Images
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ab117761, at 1/150 dilution, staining TBLR1/TBL1XR1 in HeLa cells by Immunocytochemistry. This image was produced with antibody produced in ascites.
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All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 2 : CEM cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Predicted band size: 56 kDa -
All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution
Lane 1 : Mouse Brain Lysate
Lane 2 : Mouse Heart Lysate
Lane 3 : K562 cell Lysate
Predicted band size: 56 kDaThis image was produced with antibody produced in ascites.
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ab117761, at 1/500 - 1/1000 dilution, staining TBLR1/TBL1XR1 in paraffin-embedded Human breast cancer by Imunohistochemistry. This image was produced with antibody produced in ascites.
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Immunocytochemical staining of HeLa cells using anti-TBLR1/TBL1XR1 antibody (ab117761) at a dilution of 1:200. Cells were fixed with 4% paraformaldehyde. This image was produced with antibody produced in ascites.
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Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution + K562 whole cell lysate at 40 µg
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse brain lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab117761 has been referenced in 2 publications.
- Zhou Q et al. Transducin (ß)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway. Oncogene 36:1873-1886 (2017). WB, IF . PubMed: 27694893
- Ma M & Yu N Over-Expression of TBL1XR1 Indicates Poor Prognosis of Serous Epithelial Ovarian Cancer. Tohoku J Exp Med 241:239-247 (2017). PubMed: 28344213