Overview

  • Product name
  • Description
    Rabbit polyclonal to TBLR1
  • Specificity
    Does not cross-react with TBL1.
  • Tested applications
    Suitable for: ChIP, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • HeLa nuclear extract

Properties

Applications

Our Abpromise guarantee covers the use of ab24550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 56 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 24446484

Target

  • Function
    F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the WD repeat EBI family.
    Contains 1 F-box-like domain.
    Contains 1 LisH domain.
    Contains 8 WD repeats.
  • Domain
    The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C21 antibody
    • DC42 antibody
    • F box like/WD repeat containing protein TBL1XR1 antibody
    • F-box-like/WD repeat-containing protein TBL1XR1 antibody
    • FLJ12894 antibody
    • IRA1 antibody
    • Nuclear receptor corepressor/HDAC3 complex subunit antibody
    • Nuclear receptor corepressor/HDAC3 complex subunit TBLR1 antibody
    • TBL1 related protein 1 antibody
    • TBL1-related protein 1 antibody
    • TBL1R_HUMAN antibody
    • TBL1XR1 antibody
    • Transducin (beta) like 1 X linked receptor 1 antibody
    • Transducin beta like 1X related protein 1 antibody
    • Transducin beta-like 1X-related protein 1 antibody
    see all

Images

  • Anti-TBLR1 antibody (ab24550) at 1/2000 dilution + HeLa nuclear extract

    Predicted band size : 56 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab24550 used in Immunoprecipitation.
    Lysate prepared from human lung carcinoma cells was loaded at 500µg. The immunoprecipitation step was performed using Protein G. 50% of the immunoprecipitated material was loaded on each lane. Membrane was blocked with blocking buffer (PBS+ 5% non fat milk powder) for 1 hour at room temperature followed by incubation with ab24550 in blocking buffer plus 0.1% Tween-20 overnight (around 16 hours) at 4 degree centigrade. Blot was washed thrice in PBS plus 0.1% Tween-20, followed by incubation with anti-rabbit IgG HRPO in blocking buffer plus 0.1% Tween-20 at room temperature for 1 hour. Blot was washed thrice in PBS plus 0.1% Tween-20, followed by ECL detection of reactive bands.

    See Abreview

References

This product has been referenced in:
  • Moretti C  et al. SLY regulates genes involved in chromatin remodeling and interacts with TBL1XR1 during sperm differentiation. Cell Death Differ 24:1029-1044 (2017). WB, IP ; Mouse . Read more (PubMed: 28475176) »
  • Daniels G  et al. Cytoplasmic, full length and novel cleaved variant, TBLR1 reduces apoptosis in prostate cancer under androgen deprivation. Oncotarget 7:39556-39571 (2016). WB . Read more (PubMed: 27127173) »

See all 7 Publications for this product

Customer reviews and Q&As

Application
ChIP
Detection step
Semiquantitative PCR
Sample
Mouse Tissue lysate - whole (Mouse liver)
Specification
Mouse liver
Negative control
IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37%FORMALDEHYDE
Positive control
POL ll
Username

Mr. Natarajan Balasubramaniyan

Verified customer

Submitted Sep 04 2013

This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ChIP of rat samples and include this code in the “Additional Comments” section so we know the Abreview is for this promoti...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (lung carcinoma)
Total protein in input
500 µg
Specification
lung carcinoma
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

Submitted Jun 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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