Overview

  • Product nameAnti-TBLR1 antibody - ChIP Grade
    See all TBLR1 primary antibodies
  • Description
    Rabbit polyclonal to TBLR1 - ChIP Grade
  • SpecificityDoes not cross-react with TBL1.
  • Tested applicationsSuitable for: ChIP, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • HeLa nuclear extract

Applications

Our Abpromise guarantee covers the use of ab24550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 56 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 24446484

Target

  • FunctionF-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the WD repeat EBI family.
    Contains 1 F-box-like domain.
    Contains 1 LisH domain.
    Contains 8 WD repeats.
  • DomainThe F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C21 antibody
    • DC42 antibody
    • F box like/WD repeat containing protein TBL1XR1 antibody
    • F-box-like/WD repeat-containing protein TBL1XR1 antibody
    • FLJ12894 antibody
    • IRA1 antibody
    • Nuclear receptor corepressor/HDAC3 complex subunit antibody
    • Nuclear receptor corepressor/HDAC3 complex subunit TBLR1 antibody
    • TBL1 related protein 1 antibody
    • TBL1-related protein 1 antibody
    • TBL1R_HUMAN antibody
    • TBL1XR1 antibody
    • Transducin (beta) like 1 X linked receptor 1 antibody
    • Transducin beta like 1X related protein 1 antibody
    • Transducin beta-like 1X-related protein 1 antibody
    see all

Anti-TBLR1 antibody - ChIP Grade images

  • Anti-TBLR1 antibody - ChIP Grade (ab24550) at 1/2000 dilution + HeLa nuclear extract

    Predicted band size : 56 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab24550 used in Immunoprecipitation.
    Lysate prepared from human lung carcinoma cells was loaded at 500µg. The immunoprecipitation step was performed using Protein G. 50% of the immunoprecipitated material was loaded on each lane. Membrane was blocked with blocking buffer (PBS+ 5% non fat milk powder) for 1 hour at room temperature followed by incubation with ab24550 in blocking buffer plus 0.1% Tween-20 overnight (around 16 hours) at 4 degree centigrade. Blot was washed thrice in PBS plus 0.1% Tween-20, followed by incubation with anti-rabbit IgG HRPO in blocking buffer plus 0.1% Tween-20 at room temperature for 1 hour. Blot was washed thrice in PBS plus 0.1% Tween-20, followed by ECL detection of reactive bands.

    See Abreview

References for Anti-TBLR1 antibody - ChIP Grade (ab24550)

This product has been referenced in:
  • Toret CP  et al. A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion. J Cell Biol 204:265-79 (2014). ICC/IF, WB ; Drosophila melanogaster . Read more (PubMed: 24446484) »
  • Landt SG  et al. ChIP-seq guidelines and practices of the ENCODE and modENCODE consortia. Genome Res 22:1813-31 (2012). IP . Read more (PubMed: 22955991) »

See all 5 Publications for this product

Product Wall

Application ChIP
Detection step Semiquantitative PCR
Sample Mouse Tissue lysate - whole (Mouse liver)
Specification Mouse liver
Negative control IgG
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37%FORMALDEHYDE
Positive control POL ll
Username

Mr. Natarajan Balasubramaniyan

Verified customer

Submitted Sep 04 2013

This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ChIP of rat samples and include this code in the “Additional Comments” section so we know the Abreview is for this promoti...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (lung carcinoma)
Total protein in input 500 µg
Specification lung carcinoma
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Jun 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"