Anti-TBP like protein TLP antibody (ab52772)

Overview

  • Product nameAnti-TBP like protein TLP antibody
    See all TBP like protein TLP primary antibodies
  • Description
    Mouse polyclonal to TBP like protein TLP
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Chicken
    Predicted to work with: Mouse, Human, Xenopus laevis, Zebrafish, Cynomolgus Monkey
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 116-136 of Chicken like protein TLP from Swiss Prot Q9YGV8. Target sequence used to make the antibody:

    RLPEFTKNNRPHASYEPELH

    , corresponding to internal sequence amino acids 116-136 of Chicken TBP like protein TLP

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum.
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 21 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

Anti-TBP like protein TLP antibody images

  • All lanes : Anti-TBP like protein TLP antibody (ab52772) at 1/1000 dilution

    Lane 1 : Total protein extract from E coli with ~50ng to 100ng of a fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen (his-tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution

    Predicted band size : 21 kDa

References for Anti-TBP like protein TLP antibody (ab52772)

ab52772 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"