Overview

  • Product nameAnti-Tbp7 antibody [TBP7-27]
    See all Tbp7 primary antibodies
  • Description
    Mouse monoclonal [TBP7-27] to Tbp7
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • Tested with rabbit 26S proteasome preparation.

Properties

Applications

Our Abpromise guarantee covers the use of ab22634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: 1/1000 (PMID 18986984).
    WB: 1/1000 - 1/2500. Detects a band of approximately 50 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
    • Sequence similaritiesBelongs to the AAA ATPase family.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 26S protease regulatory subunit 6B antibody
      • 26S proteasome AAA ATPase subunit RPT3 antibody
      • 26S proteasome AAA-ATPase subunit RPT3 antibody
      • MB67 interacting protein antibody
      • MB67-interacting protein antibody
      • MIP224 antibody
      • Protease 26S subunit 6 antibody
      • Proteasome (prosome macropain) 26S subunit ATPase 4 antibody
      • Proteasome 19S S6 antibody
      • Proteasome 26S subunit ATPase 4 antibody
      • Proteasome 26S subunit, ATPase, 4 antibody
      • PRS6B_HUMAN antibody
      • PSMC4 antibody
      • RPT3 antibody
      • S6 antibody
      • Tat binding protein 7 antibody
      • TAT-binding protein 7 antibody
      • TBP 7 antibody
      • TBP-7 antibody
      see all

    Anti-Tbp7 antibody [TBP7-27] images

    • Lane 1 : Anti-Tbp7 antibody [TBP7-27] (ab22634) at 1/1000 dilution (rabbit 26S proteasome preparation)
      Lane 2 : Anti-Tbp7 antibody [TBP7-27] (ab22634) at 1/2500 dilution (rabbit 26S proteasome preparation)

      Lane 1 : As above
      Lane 2 : As above


      Performed under reducing conditions.

      Observed band size : 50 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute
    • ab22634 staining Tbp7 in mock transfected rat primary neurons by Immunocytochemistry/ Immunofluorescence. Primary cultures of rat hippocampal neurons were prepared from E18 rats. Cells were fixed in 4% paraformaldehyde and incubated with primary antibody at 1/1000 dilution. The image show red staining with ab22634, nucleus was stained blue with Hoechst H33258 and right image show merged images. The green color seen in right image is the GFP staining in similar experiment.

    References for Anti-Tbp7 antibody [TBP7-27] (ab22634)

    This product has been referenced in:
    • Wilson CH  et al. Increased expression of peroxiredoxin 1 and identification of a novel lipid-metabolizing enzyme in the early phase of liver ischemia reperfusion injury. Proteomics 11:4385-96 (2011). WB ; Rat . Read more (PubMed: 21905220) »
    • Rousseau E  et al. Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones. J Biol Chem 284:1917-29 (2009). WB, ICC/IF ; Human, Rat . Read more (PubMed: 18986984) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab22634.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"