• Product nameAnti-TBR2 / Eomes antibody
    See all TBR2 / Eomes primary antibodies
  • Description
    Rabbit polyclonal to TBR2 / Eomes
  • Tested applicationsSuitable for: IHC-FoFr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the N-terminal region of human TBR2/ Eomes.

  • Positive control
    • 293 cell lysates transiently transfected with the TBR2 / Eomes gene.



Our Abpromise guarantee covers the use of ab75720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/500. PubMed: 22991445
WB 1/50 - 1/1000. Predicted molecular weight: 73 kDa.
ELISA 1/1000.


  • FunctionFunctions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
  • Tissue specificityExpressed in CD8+ T-cells.
  • Involvement in diseaseNote=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum.
  • Sequence similaritiesContains 1 T-box DNA-binding domain.
  • Developmental stageDetected at 7 weeks of development in the forebrain floorplate of the CNS. Expressed within the mantle layer and migrating neuroblasts of the telencephalon at 12.5 weeks of development.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • eomes antibody
    • EOMES_HUMAN antibody
    • Eomesodermin antibody
    • Eomesodermin homolog antibody
    • t box brain 2 antibody
    • T-box brain protein 2 antibody
    • T-brain-2 antibody
    • Tbr 2 antibody
    • TBR-2 antibody
    see all

Anti-TBR2 / Eomes antibody images

  • All lanes : Anti-TBR2 / Eomes antibody (ab75720) at 1/1000 dilution

    Lane 1 : Nontransfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the TBR2 / Eomes gene

    Lysates/proteins at 2 µg per lane.

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 73 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa,65 kDa,72 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

References for Anti-TBR2 / Eomes antibody (ab75720)

This product has been referenced in:
  • Tan SL  et al. Essential roles of the histone methyltransferase ESET in the epigenetic control of neural progenitor cells during development. Development 139:3806-16 (2012). IHC-Fr, WB ; Mouse . Read more (PubMed: 22991445) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (embryonic brain E14.5)
Permeabilization Yes - 0.3% Triton X
Specification embryonic brain E14.5
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 23 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
Sample Mouse Tissue sections (embryonic brain E14.5)
Specification embryonic brain E14.5
Permeabilization Yes - PBS/0.2% Triton X-100
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Sep 26 2014

Application Immunocytochemistry
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Mouse Cell (Embryo brain section)
Specification Embryo brain section
Permeabilization Yes - 0.2% TritonX
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted May 06 2014