Overview

  • Product name
  • Description
    Rabbit polyclonal to TBX1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 334-364 of Human TBX1

  • Positive control
    • HepG2 cell lysate, Human Testis Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab103127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 43 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/10 - 1/50.

Target

  • Function
    Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries.
  • Involvement in disease
    Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS) [MIM:188400, 192430]. DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life.
    Defects in TBX1 are a cause of DiGeorge syndrome (DGS) [MIM:188400].
    Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS) [MIM:192430].
    Defects in TBX1 are a cause of conotruncal heart malformations (CTHM) [MIM:217095]. CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies.
  • Sequence similarities
    Contains 1 T-box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brachyury antibody
    • CAFS antibody
    • CTHM antibody
    • DGCR antibody
    • DGS antibody
    • DORV antibody
    • T box antibody
    • T box 1 antibody
    • T box 1 transcription factor antibody
    • T box 1 transcription factor C antibody
    • T box protein 1 antibody
    • T box transcription factor TBX 1 antibody
    • T box transcription factor TBX1 antibody
    • T-box protein 1 antibody
    • T-box transcription factor TBX1 antibody
    • TBX 1 antibody
    • TBX 1C antibody
    • tbx1 antibody
    • TBX1_HUMAN antibody
    • TBX1C antibody
    • Testis specific T box protein antibody
    • Testis-specific T-box protein antibody
    • TGA antibody
    • VCFS antibody
    see all

Images

  • Immunofluorescence of A2058 cells labelling TBX1 with ab103127. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab103127 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBX1 immunoreactivity is localized to the nucleus.

  • Anti-TBX1 antibody (ab103127) at 1/100 dilution + HepG2 cell lysate at 35 µg

    Predicted band size : 43 kDa
  • Formalin-fixed and paraffin-embedded Human testis tissue, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining, using ab103127 at a dilution of 1/50.

References

ab103127 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab103127.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up