Overview

  • Product name
  • Description
    Rabbit polyclonal to TBX1
  • Tested applications
    Suitable for: WB, IHC-Fr, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TBX1.

    (Peptide available as ab19624.)

  • Positive control
    • WB: C3H/10T1/2 whole cell lysate. ICC/IF: U-2 OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab18530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 21908517
IP Use at an assay dependent concentration. PubMed: 25197075
IHC-P Use at an assay dependent concentration. PubMed: 21177346
ICC/IF 1/100.

Target

  • Function
    Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries.
  • Involvement in disease
    Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS) [MIM:188400, 192430]. DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life.
    Defects in TBX1 are a cause of DiGeorge syndrome (DGS) [MIM:188400].
    Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS) [MIM:192430].
    Defects in TBX1 are a cause of conotruncal heart malformations (CTHM) [MIM:217095]. CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies.
  • Sequence similarities
    Contains 1 T-box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brachyury antibody
    • CAFS antibody
    • CTHM antibody
    • DGCR antibody
    • DGS antibody
    • DORV antibody
    • T box antibody
    • T box 1 antibody
    • T box 1 transcription factor antibody
    • T box 1 transcription factor C antibody
    • T box protein 1 antibody
    • T box transcription factor TBX 1 antibody
    • T box transcription factor TBX1 antibody
    • T-box protein 1 antibody
    • T-box transcription factor TBX1 antibody
    • TBX 1 antibody
    • TBX 1C antibody
    • tbx1 antibody
    • TBX1_HUMAN antibody
    • TBX1C antibody
    • Testis specific T box protein antibody
    • Testis-specific T-box protein antibody
    • TGA antibody
    • VCFS antibody
    see all

Anti-TBX1 antibody images

  • All lanes : Anti-TBX1 antibody (ab18530) at 1 µg/ml

    Lane 1 : C3H/10T1/2 (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : C3H/10T1/2 (mouse embryonic fibroblast cell line) whole cell lysate with Human TBX1 peptide (ab19624) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    ab18530 recognizes a single band at ~50kDa in the mouse embryonic fibroblast cell line C3H/10T1/2 (lane 1), that approximately corresponds in size to the given molecular weight of TBX1 of 43kDa. The addition of the immunizing peptide (lane 2) blocks the interaction of the antibody with this protein, suggesting that this is a specific interaction.
  • ab18530 staining U-2 OS (human bone osteosarcoma epithelial cell line) cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton-100 [PBS] prior to blocking in 1% BSA for 15 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C.  The secondary antibody was an Alexa Fluor® 647 conjugated donkey anti-rabbit, diluted 1/1000.

    See Abreview

References for Anti-TBX1 antibody (ab18530)

This product has been referenced in:
  • Bain VE  et al. Tissue-specific roles for sonic hedgehog signaling in establishing thymus and parathyroid organ fate. Development 143:4027-4037 (2016). Read more (PubMed: 27633995) »
  • Reeh KA  et al. Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis. Development 141:2950-8 (2014). Read more (PubMed: 25053428) »

See all 11 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis (human mouse and rat))
Specification
Testis (human mouse and rat)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
sequential peroxidase and protein block (prediluted) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Jun 04 2008

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Cell lysate - whole cell (PC12)
Total protein in input
400 µg
Specification
PC12
Immuno-precipitation step
Protein A
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 28 2008

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat, Ms Testis, Ms Lung, PC12, 9L)
Loading amount
20 µg
Specification
Jurkat, Ms Testis, Ms Lung, PC12, 9L
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X-100 [PBS]
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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