Recombinant Anti-TBX1 antibody [EPR3289(2)] (ab109313)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3289(2)] to TBX1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-TBX1 antibody [EPR3289(2)]
See all TBX1 primary antibodies -
Description
Rabbit monoclonal [EPR3289(2)] to TBX1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Jurkat, NCCIT, PC-12, F9, C6, Neuro-2a, HEK-293, HeLa cell lysates
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3289(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
Target
-
Function
Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries. -
Involvement in disease
Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS) [MIM:188400, 192430]. DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life.
Defects in TBX1 are a cause of DiGeorge syndrome (DGS) [MIM:188400].
Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS) [MIM:192430].
Defects in TBX1 are a cause of conotruncal heart malformations (CTHM) [MIM:217095]. CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies. -
Sequence similarities
Contains 1 T-box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 6899 Human
- Entrez Gene: 21380 Mouse
- Entrez Gene: 360737 Rat
- Omim: 602054 Human
- SwissProt: O43435 Human
- SwissProt: P70323 Mouse
- Unigene: 173984 Human
- Unigene: 295194 Mouse
-
Alternative names
- Brachyury antibody
- CAFS antibody
- CTHM antibody
see all
Images
-
All lanes : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/1000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) control cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) nuclear cell lysate
Lane 3 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : Human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking buffer: 5% NFDM/TBST.
There is a strong non-specific band in human liver. Human liver is negative control for TBX1 which is consistent with PMID: 28920943.
-
All lanes : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/2000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking buffer: 5% NFDM/TBST.
-
Lane 1 : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/2000 dilution
Lane 2 : Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/5000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST.
Exposure time: 30 seconds (Lane 1) and 180 seconds (Lane 2).
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab109313 has been referenced in 9 publications.
- Lin QY et al. VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis. Clin Transl Med 11:e374 (2021). PubMed: 33783987
- Hou L et al. Dihydromyricetin Ameliorates Inflammation-Induced Insulin Resistance via Phospholipase C-CaMKK-AMPK Signal Pathway. Oxid Med Cell Longev 2021:8542809 (2021). PubMed: 34650665
- Hou L et al. Dihydromyricetin resists inflammation-induced muscle atrophy via ryanodine receptor-CaMKK-AMPK signal pathway. J Cell Mol Med 25:9953-9971 (2021). PubMed: 34676967
- Meyers K et al. Lipocalin-2 deficiency may predispose to the progression of spontaneous age-related adiposity in mice. Sci Rep 10:14589 (2020). PubMed: 32883997
- Cui J et al. TBX1 Functions as a Tumor Activator in Prostate Cancer by Promoting Ribosome RNA Gene Transcription. Front Oncol 10:616173 (2020). PubMed: 33575219
- Li C et al. MicroRNA-3651 promotes colorectal cancer cell proliferation through directly repressing T-box transcription factor 1. Int J Mol Med 45:956-966 (2020). PubMed: 31922246
- Velazquez-Villegas LA et al. TGR5 signalling promotes mitochondrial fission and beige remodelling of white adipose tissue. Nat Commun 9:245 (2018). PubMed: 29339725
- Hou L et al. Browning of Pig White Preadipocytes by Co-Overexpressing Pig PGC-1a and Mice UCP1. Cell Physiol Biochem 48:556-568 (2018). PubMed: 30021212
- Yu Y et al. Smad7 enables STAT3 activation and promotes pluripotency independent of TGF-ß signaling. Proc Natl Acad Sci U S A 114:10113-10118 (2017). PubMed: 28874583