Overview

  • Product name
  • Description
    Rabbit polyclonal to TBX18
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Chimpanzee, Zebrafish, Spider monkey
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids 504-553 (SSNAFATNQT HQGSYNTFRL HSPCALYGYN FSTSPKLAAS PEKIVSSQGS) of Human TBX18 (NP_001073977.1).

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

Target

Images

  • Anti-TBX18 antibody (ab86332) at 1 µg/ml (5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of paraffin embedded renal tubule tissue, tested with an antibody dilution of 5 µg/ml (ab86332)

  • Immunohistochemistry of paraffin embedded breast tissue, tested with an antibody dilution of 5 µg/ml (ab86332)

References

ab86332 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab86332.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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