Overview

  • Product name
  • Description
    Rabbit polyclonal to Tbx2
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Tbx2.

    (Peptide available as ab35983.)

  • Positive control
    • This antibody gave a positive signal in the following Human tissue lysates: Kidney; Lung; Heart

Properties

Applications

Our Abpromise guarantee covers the use of ab33298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-Tbx2 antibody (ab33298) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Lung (Human) Tissue Lysate
    Lane 3 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
  • Formalin-fixed paraffin-embedded mouse bone marrow section stained with ab33298 Tbx2 antibody at 1/500 dilution.
  • ab33298 staining Tbx2 in mouse heart and somatopleure tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with methanol. Samples were then blocked with 5% serum for 2 hours at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An undiluted Cy2®-conjugated Donkey anti-rabbit IgG was used as secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Liu F  et al. TBX2 expression is regulated by PAX3 in the melanocyte lineage. Pigment Cell Melanoma Res 26:67-77 (2013). Read more (PubMed: 23020925) »
  • Lüdtke TH  et al. Tbx2 controls lung growth by direct repression of the cell cycle inhibitor genes Cdkn1a and Cdkn1b. PLoS Genet 9:e1003189 (2013). Read more (PubMed: 23341776) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart and somatopleure)
Specification
Heart and somatopleure
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Jun Xu

Verified customer

Submitted May 25 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bone Marrow)
Specification
Bone Marrow
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Apr 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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