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Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Tbx2.
(Peptide available as ab35983.)
Our Abpromise guarantee covers the use of ab33298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
|IHC-P||1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
ab33298 staining Tbx2 in mouse heart and somatopleure tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with methanol. Samples were then blocked with 5% serum for 2 hours at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An undiluted Cy2®-conjugated Donkey anti-rabbit IgG was used as secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"