• Product nameAnti-Tbx3 antibody
    See all Tbx3 primary antibodies
  • Description
    Mouse monoclonal to Tbx3
  • Tested applicationsSuitable for: Flow Cyt, WB, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Macaque monkey
  • Immunogen

    Recombinant fragment corresponding to amino acids 311 - 411 (KENGTSDESS SEQAAFNCFA QASSPAASTV GTSNLKDLCP SEGESDAEAE SKEEHGPEAC DAAKISTTTS EEPCRDKGSP AVKAHLFAAE RPRDSGRLDK *) of Human Tbx3 (NP_005987), linked to a proprietary tag

  • Positive control
    • HepG2 cells



Our Abpromise guarantee covers the use of ab89220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 79 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 23082988


Anti-Tbx3 antibody images

  • Anti-Tbx3 antibody (ab89220) at 5 µg/ml + immunogen (100aa protein fragment with a proprietary tag of 26kDa) at 0.2 µg

    Predicted band size : 79 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-Tbx3 antibody (ab89220) at 5 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 79 kDa
  • ab89220 at 10 µg/ml staining Tbx3 in paraformaldehyde fixed, permeabilized HepG2 cells by immunofluorescence.
  • Overlay histogram showing HeLa cells stained with ab89220 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89220, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Tbx3 antibody (ab89220)

This product has been referenced in:
  • Burgucu D  et al. Tbx3 represses PTEN and is over-expressed in head and neck squamous cell carcinoma. BMC Cancer 12:481 (2012). IHC-P, Flow Cyt ; Human . Read more (PubMed: 23082988) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (h9 cell line upon differentiation)
Specification h9 cell line upon differentiation
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 22 2014

Thank you for your technical support and interest in our products.

As the datasheet indicates, the recommended positive control for ab89220: Anti-Tbx3 antibody is HepG2 cell lysate for Western blot application.

I have conducted a se...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic stem cells)
Loading amount 400000 cells
Specification embryonic stem cells
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Kate Hawkins

Verified customer

Submitted Feb 22 2011

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart and forelimb)
Specification Heart and forelimb
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%

Mr. Jun Xu

Verified customer

Submitted May 28 2010