Anti-TBX5 antibody (ab137833)

Overview

  • Product name
  • Description
    Rabbit polyclonal to TBX5
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-243 of Human TBX5 (UniProt ID: Q99593).

  • Positive control
    • HeLa and HepG2 whole cell lysates; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab137833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
ICC/IF 1/100 - 1/1000.

Target

Anti-TBX5 antibody images

  • Anti-TBX5 antibody (ab137833) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 58 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling TBX5 using ab137833 at a 1/500 dilution.
    Lower image is costained with Hoechst 33342.

References for Anti-TBX5 antibody (ab137833)

ab137833 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Limb Tissue)
Permeabilization
Yes - 1% Triton X-100
Specification
Limb Tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Gufa Lin

Verified customer

Submitted Apr 10 2017

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Embryos)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Treatment
hydroxyurea
Specification
Embryos
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Nazem El Husseini

Verified customer

Submitted May 16 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocytes)
Specification
Human pluripotent stem cell derived cardiomyocytes
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 28 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (E12.5 Sternum)
Specification
E12.5 Sternum
Username

Ms. Sana Zakaria

Verified customer

Submitted Jan 28 2013

Thank you for contacting us. At this time, we do not have any feedback regarding the use of ab137833 on wholemount IHC. Unfortunately, we do not provide small or trial sized samples of any of our products. Under our new Abtrial program (which has repla...

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