Anti-TBX5 antibody (ab49308)

Overview

  • Product nameAnti-TBX5 antibody
    See all TBX5 primary antibodies
  • Description
    Rabbit polyclonal to TBX5
  • SpecificityThis antibody is specific for TBX5.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide found within the following sequence: NSMHKYQPRL HIVKADENNG FGSKNTAFCT HVFPETAFIA VTSYQNHKIT (Human)

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-TBX5 antibody images

  • Lane 1 : Marker
    Lane 2 : Anti-TBX5 antibody (ab49308) at 5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg/ml

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 53 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of Human Brain, cortex tissue at an antibody concentration of 5.0µg/ml using anti-TBX5 antibody (ab49308)

References for Anti-TBX5 antibody (ab49308)

ab49308 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification Human pluripotent stem cell derived cardiomyocyte
Permeabilization Yes - saponin
Fixative Paraformaldehyde
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Submitted Aug 27 2014

Thank you for your question. The peptide immunogen for ab49308 has 100% homology to the Scyliorhinus canicula T-box protein 5 (Tbx5) sequence you provided. I hope this is helpful. Please contact me again if you have any further questions.

Thank you for your enquiry. Thus far I have found the following information regarding potential reactivity of our TBX5 antibodies to Scyliorhinus canicula: ab58253 - no chance for cross reactivity ab86370 - region of immunogen overlaps partially but th...

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Please find below two links to our recommended protocols: One details a full nuclear extraction protocol http://www.abcam.com/ps/pdf/protocols/Nuclear%20fractionation%20protocol.pdf one details the extraction using RIPA buffer:http://www.abcam...

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Thank you for your enquiry. I am sorry to hear you are having a problem with ab49308. As you mention, this antibody has so far not been tested in zebrafish but we would expect the antibody to work in this species due to the homology of the protein i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"